Live-Cell Imaging of Sirtuin Activity Using a One-Step Fluorescence Probe
Live-Cell Imaging of Sirtuin Activity Using a One-Step Fluorescence Probe
复制标题
使用一步式荧光探针对 Sirtuin 活性进行活细胞成像
DOI：
10.1007/978-1-0716-1258-3_14
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发表时间：
2021
影响因子：
--
通讯作者：
Hidehiko Nakagawa
中科院分区：
文献类型：
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作者：
Mitsuyasu Kawaguchi;Hidehiko Nakagawa
Sirtuins（SIRTs）是NAD+依赖性组蛋白脱乙酰酶（HDACs）家族。在哺乳动物中，SIRTs功能障碍与年龄相关的代谢疾病、癌症甚至衰老有关。因此，在活细胞或组织中检测SIRT活性将有助于诊断广泛的SIRT相关疾病。在这里，我们提出的方法来测量SIRT活性在活细胞中使用我们新开发的SIRT荧光探针，KST-F-DA。
Sirtuins (SIRTs) are a family of NAD+-dependent histone deacetylases (HDACs). In mammals, dysfunction of SIRTs is associated with age-related metabolic diseases, cancers, and even aging. Therefore, the detection of SIRT activity in living cells or tissues would be helpful for diagnosis of a wide range of SIRT-associated diseases. Here, we present methodology to measure SIRT activity in living cells by using our newly developed SIRT fluorescence probe, KST-F-DA.