Circulating polyribophosphate in Hemophilus influenzae, type b meningitis. Correlation with clinical course and antibody response.
Circulating polyribophosphate in Hemophilus influenzae, type b meningitis. Correlation with clinical course and antibody response.
复制标题
B 型脑膜炎流感嗜血杆菌中的循环聚核糖磷酸。
DOI：
10.1172/jci108148
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发表时间：
1975
期刊：
影响因子：
--
通讯作者：
D. Smith
中科院分区：
文献类型：
--
作者：
R. O'Reilly;P. Anderson;D. Ingram;G. Peter;D. Smith
在由B型流感嗜血杆菌引起的全身感染中，荚膜多糖多核糖磷酸盐被释放到循环中。对45例H.流感病毒，B型脑膜炎，通过放射性标记的抗原结合抑制试验。在急性血清和脑脊液样本中经常发现多核糖磷酸盐，并且在开始有效治疗后1-30天内可以以未结合的形式检测到。在17例患者的血清样品中检测到与酸和胃蛋白酶可解离的多核糖磷酸复合物，其中一例在住院后145天内检测到。研究了多核糖磷酸水平和清除模式与住院病程和抗体反应的关系。长期抗原血症的患者在住院期间有长期发热和严重的神经系统症状，经常与局灶性并发症。在79%的研究患者，包括60%的儿童1岁或以下的年龄在第一个100天的恢复期通过放射免疫法检测抗多聚核糖磷酸抗体反应。抗体应答的强度虽然与患者的年龄明显相关，但通过抗原清除效率更可靠地预测。长期抗原血症患者的抗体应答均较低，与年龄无关。脑膜炎后未能产生多聚核糖磷酸抗体的患者也表现出抗原清除受损。这些研究表明，清除多核糖磷酸盐所需的机制可能会影响体液免疫反应的发展和强度，并增加婴儿和儿童清除系统发育缺陷的可能性。
In systemic infections caused by Hemophilus influenzae, type b, the capsular polysaccharide, polyribophosphate, is released into the circulation. Polyribophosphate was quantitated in serial serum and cerebrospinal fluid samples from 45 children with H. influenzae, type b meningitis by means of a radiolabeled antigen-binding inhibition assay. Polyribophosphate was regularly found in acute serum and cerebrospinal fluid samples and could be detected in unbound form for periods of 1-30 days after initiation of effective therapy. Complexes of polyribophosphate dissociable with acid and pepsin were detected in serum samples from 17 patients, in one case for a period of 145 days after hospitalization. Polyribophosphate levels and patterns of clearance were studied in relation to hospital course and antibody response. Patients with prolonged antigenemia had protracted fevers and severe neurological symptoms during hospitalization, frequently with focal complications.Antipolyribophosphate antibody responses were detected during the first 100 days of convalescence by radioimmunoassay in 79% of the patients studied, including 60% of the children 1 yr or less in age. The intensity of antibody response although clearly related to the age of the patient, was more reliably predicted by the efficiency of antigen clearance. Antibody responses were uniformly of low magnitude in patients with prolonged antigenemia, irrespective of age. Paients who failed to develop antibody to polyribophosphate after meningitis also exhibited impaired antigen clearance. These studies suggest that mechanisms necessary for clearance of polyribophosphate may influence the development and intensity of the humoral immune response and raise the possibility of developmental deficiencies in the clearance system in infants and children.