The neuroprotective effect of pyrroloquinoline quinone on traumatic brain injury.
The neuroprotective effect of pyrroloquinoline quinone on traumatic brain injury.
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DOI：
10.1089/neu.2011.1882
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发表时间：
2012-03
影响因子：
4.2
通讯作者：
Lili Zhang;Jie Liu;Chun Cheng;Ying Yuan;Biyun Yu;A. Shen;Meijuan Yan
中科院分区：
文献类型：
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作者：
Lili Zhang;Jie Liu;Chun Cheng;Ying Yuan;Biyun Yu;A. Shen;Meijuan Yan
吡咯喹啉醌（PQQ）是一种水溶性阴离子类醌物质，已被确定为动物必需营养素。由于PQQ在各种系统中作为抗氧化剂和氧化还原调节剂的固有特性，PQQ有望在不久的将来用于药理学应用。虽然近年来有许多研究研究了PQQ的神经保护作用，但PQQ对创伤性脑损伤（TBI）的作用尚未得到证实。在本研究中，我们采用Morris水迷宫（MWM）训练，结果表明PQQ可以改善脑外伤后动物的行为表现。考虑到许多实验表明β-1,4-半乳糖转移酶I （β-1,4- galt -I）和-V在炎症和神经系统中发挥重要作用，本研究我们采用Western blot分析研究PQQ对β-1,4- galt -I和-V表达的影响。我们发现PQQ系统给药后β-1,4- galt - i和-V的表达明显上调。rca -1凝集素荧光染色也显示PQQ参与了半乳糖苷酶β-1 （Gal β-1）、4-半乳糖转移酶n-酰基鞘氨酸（4-GlcNAc）组在皮质和海马CA2区小胶质细胞和神经元中的表达上调。综上所述，我们的实验表明PQQ可能在脑外伤后的恢复中发挥重要作用。
Pyrroloquinoline quinone (PQQ) is a water-soluble, anionic, quinonoid substance that has been established as an essential nutrient in animals. Owing to the inherent properties of PQQ as an antioxidant and redox modulator in various systems, PQQ is expected to be used in pharmacological applications in the near future. Although many recent studies have investigated its neuroprotective effects, the effect of PQQ on traumatic brain injury (TBI) has not been examined. In this study we employed Morris water maze (MWM) training, the results of which showed that PQQ led to improved behavioral performance in post-TBI animals. Considering that many experiments have suggested that β-1,4-galactosyltransferase I (β-1,4-GalT-I) and -V play significant roles in inflammation and the nervous system, in the present study we used Western blot analysis to study the effect of PQQ on the expression of β-1,4-GalT-I and -V. We found apparent expression upregulation of β-1,4-GalT-I and -V after PQQ was systemically administered. Lectin-fluorescent staining with RCA-I also revealed that PQQ contributed to expression upregulation of the galactosidase β-1 (Gal β-1), 4-galactosyltransferase N-acylsphingosine (4-GlcNAc) group in microglia and neurons of the cortex and hippocampal CA2 region. In summary, our experiment established that PQQ may play an important role in recovery post-TBI.