Isolation and Characterization of Influenza C Viruses in the Philippines and Japan
Isolation and Characterization of Influenza C Viruses in the Philippines and Japan
复制标题
DOI：
10.1128/jcm.02628-14
复制
发表时间：
2015-03-01
影响因子：
9.4
通讯作者：
Oshitani, Hitoshi
中科院分区：
文献类型：
--
作者：
Odagiri, Takashi;Matsuzaki, Yoko;Oshitani, Hitoshi
2009年11月至2013年12月，在菲律宾，使用MDCK细胞从患有严重肺炎和流感样疾病(ILI)的儿童标本中分离出15种C型流感病毒。这是菲律宾首例丙型流感病毒分离报告。此外，2008年1月至2013年12月，从日本仙台市急性呼吸道疾病(ARI)患儿的标本中分离到7株C型流感病毒。用血凝素-酯酶(HE)糖蛋白的单抗进行抗原性分析，发现19株(12株来自菲律宾，7株来自日本)与C类流感病毒参考毒株C/Sao Paulo/378/82(SP82)相似。对HE基因的系统发育分析表明，来自菲律宾和日本的菌株在SP82相关谱系中形成了不同的簇。包括菲律宾和日本毒株在内的集群被证明是在1993年左右从一个共同的祖先那里分化出来的。此外，对内部基因的系统发育分析表明，在菲律宾和日本分离的所有菌株都是通过重新分类事件出现的。菲律宾株的内部基因组成与日本株不同，但通过HE基因序列分析，所有株都属于与SP82相关的谱系。这些观察表明，这里分析的C型流感病毒是通过不同的重排事件出现的，但重排事件发生的时间和地点尚未确定。
From November 2009 to December 2013 in the Philippines, 15 influenza C viruses were isolated, using MDCK cells, from specimens obtained from children with severe pneumonia and influenza-like illness (ILI). This is the first report of influenza C virus isolation in the Philippines. In addition, from January 2008 to December 2013, 7 influenza C viruses were isolated from specimens that were obtained from children with acute respiratory illness (ARI) in Sendai city, Japan. Antigenic analysis with monoclonal antibodies to the hemagglutinin-esterase (HE) glycoprotein showed that 19 strains (12 from the Philippines and 7 from Japan) were similar to the influenza C virus reference strain C/Sao Paulo/378/82 (SP82). Phylogenetic analysis of the HE gene showed that the strains from the Philippines and Japan formed distinct clusters within an SP82-related lineage. The clusters that included the Philippine and Japanese strains were shown to have diverged from a common ancestor around 1993. In addition, phylogenetic analysis of the internal genes showed that all strains isolated in the Philippines and Japan had emerged through reassortment events. The composition of the internal genes of the Philippine strains was different from that of the Japanese strains, although all strains were classified into an SP82-related lineage by HE gene sequence analysis. These observations suggest that the influenza C viruses analyzed here had emerged through different reassortment events; however, the time and place at which the reassortment events occurred were not determined.