Functional Properties of Cell-Seeded Three-Dimensionally Woven Poly(ε-Caprolactone) Scaffolds for Cartilage Tissue Engineering
Functional Properties of Cell-Seeded Three-Dimensionally Woven Poly(ε-Caprolactone) Scaffolds for Cartilage Tissue Engineering
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DOI：
10.1089/ten.tea.2009.0480
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发表时间：
2010-04-01
影响因子：
4.1
通讯作者：
Guilak, Farshid
中科院分区：
文献类型：
--
作者：
Moutos, Franklin T.;Guilak, Farshid
关节软骨具有复杂的机械性能，使健康的关节能够承受反复的载荷，并在整个生命周期内保持光滑的关节表面。在这项研究中，我们利用纤维增强复合支架设计模仿天然软骨的各向异性，非线性和粘弹性的生物力学特性作为开发功能性组织工程结构的基础。用纤维蛋白水凝胶包封三维编织的聚（ε-己内酯）（PCL）支架，接种人脂肪源性干细胞，并在软骨形成培养条件下培养28天。生物力学测试表明，PCL为基础的结构表现出基线压缩和剪切性能类似的天然软骨，并保持这些属性在整个培养期间，同时支持富含胶原蛋白的细胞外基质的合成。此外，在规定的培养期内，构建体显示出与天然关节软骨相似的平衡摩擦系数（mu（eq）类似于0.1-0.3）。我们的研究结果表明，三维编织的PCL-纤维蛋白复合支架可以产生软骨样的机械性能，这些工程特性可以保持在培养中，而种子干细胞再生一个新的，功能性的组织结构。
Articular cartilage possesses complex mechanical properties that provide healthy joints the ability to bear repeated loads and maintain smooth articulating surfaces over an entire lifetime. In this study, we utilized a fiber-reinforced composite scaffold designed to mimic the anisotropic, nonlinear, and viscoelastic biomechanical characteristics of native cartilage as the basis for developing functional tissue-engineered constructs. Three-dimensionally woven poly(epsilon-caprolactone) (PCL) scaffolds were encapsulated with a fibrin hydrogel, seeded with human adipose-derived stem cells, and cultured for 28 days in chondrogenic culture conditions. Biomechanical testing showed that PCL-based constructs exhibited baseline compressive and shear properties similar to those of native cartilage and maintained these properties throughout the culture period, while supporting the synthesis of a collagen-rich extracellular matrix. Further, constructs displayed an equilibrium coefficient of friction similar to that of native articular cartilage (mu(eq) similar to 0.1-0.3) over the prescribed culture period. Our findings show that three-dimensionally woven PCL-fibrin composite scaffolds can be produced with cartilage-like mechanical properties, and that these engineered properties can be maintained in culture while seeded stem cells regenerate a new, functional tissue construct.