The distinct and developmentally regulated patterns of expression of members of the mouse Cdc25 gene family suggest differential functions during gametogenesis.
The distinct and developmentally regulated patterns of expression of members of the mouse Cdc25 gene family suggest differential functions during gametogenesis.
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小鼠 Cdc25 基因家族成员独特的发育调控表达模式表明配子发生过程中的不同功能。
DOI：
10.1006/dbio.1995.1207
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发表时间：
1995
影响因子：
2.7
通讯作者：
Wolgemuth,DJ
中科院分区：
文献类型：
--
作者：
Wu,S;Wolgemuth,DJ
CDC25基因编码蛋白苏氨酸/酪氨酸磷酸酶，激活细胞周期蛋白依赖性激酶。在一项旨在鉴定参与调节发育中的小鼠生殖细胞有丝分裂和减数分裂细胞周期的基因的研究中，分离出了编码人CDC25C基因的小鼠同源基因。通过Northern杂交和原位杂交以及免疫印迹分析，确定了CDC25C与第二个CDC25家族成员CDC25B的表达模式。在妊娠中期胚胎和包括睾丸和卵巢在内的几个成体组织中检测到2.1kb的小鼠CDC25C转录本。在睾丸中检测到最高水平的cdc25C转录本，低水平的2.1kb转录本和丰富的1.9kb转录本，这在被检测的其他组织中没有检测到。在睾丸中，CDC25C表达定位于生殖细胞，尤其是粗线期晚期精母细胞和圆形精母细胞，而CDC25B表达最易在体细胞中检测到。在卵巢中，CDC25C在卵丘颗粒细胞中有明显的表达，而在发育中的卵母细胞和体细胞中都有表达，包括颗粒细胞。这些结果不仅表明小鼠CDC25基因在小鼠睾丸和卵巢中的表达具有谱系和发育阶段的特异性，而且还表明CDC25基因在卵巢和睾丸的生发和躯体部分可能具有不同的功能，其中只有一部分参与了细胞增殖。
Cdc25 genes encode protein threonine/tyrosine phosphatases that activate cyclin-dependent kinases. A cDNA encoding the murine homologue of the human Cdc25C gene was isolated in studies designed to identify those genes involved in regulating the mitotic and meiotic cell cycles of developing mouse germ cells. Northern blot and in situ hybridization along with immunoblot analyses were performed to determine the patterns of expression of Cdc25C compared to those of a second Cdc25 family member, Cdc25B . Murine Cdc25C transcripts of 2.1 kb were detected in midgestation embryos and in several adult tissues, including testis and ovary. The highest levels of Cdc25C transcripts were detected in the testis, with low levels of the 2.1-kb transcript and abundant levels of a 1.9-kb transcript that was not detected in the other tissues examined. In the testis, Cdc25C expression was localized in germ cells, specifically in late pachytene-diplotene spermatocytes and round spermatids, whereas Cdc25B expression was most readily detected in the somatic cells. In the ovary, Cdc25C expression was apparent in cumulus granulosa cells, whereas the expression of Cdc25B was detected in both growing oocytes and somatic cells, including the granulosa cells. These results not only indicate that the expression of the murine Cdc25 genes is lineage- and developmental stage-specific in the mouse testis and ovary but also suggest that the Cdc25 genes may have different functions in the germinal and somatic compartments of the ovary and the testis, only some of which are involved in cellular proliferation.