Differential arrangements of conserved building blocks among homologs of the Rad50/Mre11 DNA repair protein complex
Differential arrangements of conserved building blocks among homologs of the Rad50/Mre11 DNA repair protein complex
复制标题
DOI：
10.1016/j.jmb.2004.04.014
复制
发表时间：
2004-06-11
影响因子：
5.6
通讯作者：
Wyman, C
中科院分区：
文献类型：
--
作者：
de Jager, M;Trujillo, KM;Wyman, C
染色体结构维持蛋白（SMC）具有多种细胞功能，包括染色体分离、染色体浓缩和DNA修复。它们基于一组保守的不同结构基序进行分组。所有的SMC蛋白都被预测为具有一个二分ATP酶结构域，该结构域被一个长的区域分开，该区域被预测为形成卷曲螺旋。最近的各种SMC蛋白的结构数据显示，它们被安排为长的分子内卷曲螺旋与球状ATP酶在一端。SMC蛋白质作为异二聚体或同二聚体成对发挥功能，通常与其他蛋白质形成复合物。我们期望SMC蛋白结构域在复杂组装中的排列对它们的不同功能具有重要意义。我们使用扫描力显微镜成像来确定人、酿酒酵母、激烈热球菌Rad 50/Mre 11、大肠杆菌SbcCD和S.酿酒酵母SMC 1/SMC 3粘附素SMC复合物。两个不同的架构安排进行了描述，根据其组件的连接方式。真核复合物彼此相似，与原核和古细菌同源物不同。这些相似性和差异进行了讨论，就其在染色体代谢中的不同机制的作用。(C)2004爱思唯尔有限公司保留所有权利。
Structural maintenance of chromosomes (SMC) proteins have diverse cellular functions including chromosome segregation, condensation and DNA repair. They are grouped based on a conserved set of distinct structural motifs. All SMC proteins are predicted to have a bipartite ATPase domain that is separated by a long region predicted to form a coiled coil. Recent structural data on a variety of SMC proteins shows them to be arranged as long intramolecular coiled coils with a globular ATPase at one end. SMC proteins function in pairs as heterodimers or as homodimers often in complexes with other proteins. We expect the arrangement of the SMC protein domains in complex assemblies to have important implications for their diverse functions. We used scanning force microscopy imaging to determine the architecture of human, Saccharomyces cerevisiae, and Pyrococcus furiosus Rad50/Mre11, Escherichia coli SbcCD, and S. cerevisiae SMC1/SMC3 cohesin SMC complexes. Two distinct architectural arrangements are described, based on the way their components were connected. The eukaryotic complexes were similar to each other and differed from their prokaryotic and archaeal homologs. These similarities and differences are discussed with respect to their diverse mechanistic roles in chromosome metabolism. (C) 2004 Elsevier Ltd. All rights reserved.