Formulation and in-vitro characterization of retinoic acid loaded poly (lactic-co-glycolic acid) microspheres
Formulation and in-vitro characterization of retinoic acid loaded poly (lactic-co-glycolic acid) microspheres
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DOI：
10.1080/02652040500273878
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发表时间：
2005-12-01
影响因子：
3.9
通讯作者：
Hincal, AA
中科院分区：
文献类型：
--
作者：
Çirpanli, Y;Ünlü, N;Hincal, AA
采用乳液/溶剂蒸发技术制备了含有全反式视黄酸（atRA）的聚乳酸-乙醇酸（PLGA）微球。使用比浓对数粘度为0.17和0.39 dL g(-1)的PLGA (50:50)。使用聚乙烯醇 (PVA) 或 PVA 和油酸钠 (SO) 组合 (4:1) 来稳定乳液。研究了聚合物粘度、乳化剂类型和浓度对 atRA 从微球体外释放的影响。还测试了微粒在4℃、25℃和40℃温度下的稳定性。粒径范围在1-2μm之间。通过扫描电子显微镜（SEM）照片确定微球形状光滑且呈球形。微球的产率在50-75%之间，包封率在45-75%之间。体外释放研究表明，atRA 从微球中的释放可持续 11 天。
Poly (lactic-co-glycolic acid) (PLGA) microspheres containing all-trans retinoic acid (atRA) were prepared by emulsion/solvent evaporation technique. PLGA (50:50) with inherent viscosities of 0.17 and 0.39 dL g(-1) was used. Polyvinyl alcohol (PVA) or PVA and sodium oleate (SO) combinations (4:1) were used to stabilize the emulsions. The effect of polymer viscosity, emulsifier type and concentration on the in vitro release of atRA from the microspheres was investigated. The stability of the microparticles was also tested at the temperatures of 4, 25 and 40 degrees C. The particle size ranged between 1-2 mu m. Microspheres were smooth and spherical in shape, as determined by scanning electron microscope (SEM) photographs. The yield of microspheres ranged from 50-75% and the encapsulation efficiency was determined between 45-75%. In vitro release studies showed that atRA release from microspheres lasted for 11 days.