MU-CRYSTALLIN IS A MAMMALIAN HOMOLOG OF AGROBACTERIUM-ORNITHINE CYCLODEAMINASE AND IS EXPRESSED IN HUMAN RETINA
MU-CRYSTALLIN IS A MAMMALIAN HOMOLOG OF AGROBACTERIUM-ORNITHINE CYCLODEAMINASE AND IS EXPRESSED IN HUMAN RETINA
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DOI：
10.1073/pnas.89.19.9292
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发表时间：
1992-10-01
影响因子：
11.1
通讯作者：
WISTOW, GJ
中科院分区：
文献类型：
--
作者：
KIM, RY;GASSER, R;WISTOW, GJ
Mu-crystallin是几种澳大利亚有袋动物眼睛透镜的主要成分。通过cDNA克隆获得了袋鼠μ-晶体蛋白的全序列。预测的氨基酸序列与根癌农杆菌的肿瘤诱导（Ti）质粒编码的鸟氨酸环脱氨酶相似。到目前为止，在真核生物中既没有观察到鸟氨酸环脱氨酶，也没有观察到任何结构相关的酶。袋鼠组织的RNA分析显示，μ-晶状体蛋白在透镜中以高丰度表达，但在透镜外，μ-晶状体蛋白优先在神经组织、视网膜和脑中表达。从人视网膜中克隆了几乎全长的μ-晶体蛋白cDNA。在人类组织中，μ-晶状体蛋白mRNA存在于神经组织、肌肉和肾脏中。这种模式的表达和关系的酶参与不寻常的氨基酸代谢表明，哺乳动物mu-crystallin可能是酶参与的过程，如兴奋性氨基酸的调节或代谢。
Mu-crystallin is the major component of the eye lens in several Australian marsupials. The complete sequence of kangaroo mu-crystallin has now been obtained by cDNA cloning. The predicted amino acid sequence shows similarity with ornithine cyclodeaminases encoded by the tumor-inducing (Ti) plasmids of Agrobacterium tumefaciens. Until now, neither ornithine cyclodeaminase nor any structurally related enzymes have been observed in eukaryotes. RNA analysis of kangaroo tissues shows that mu-crystallin is expressed at high abundance in lens, but outside the lens mu-crystallin is preferentially expressed in neural tissues, retina, and brain. An almost full-length cDNA for mu-crystallin was cloned from human retina. In human tissues, mu-crystallin mRNA is present in neural tissue, muscle, and kidney. This pattern of expression and relationship to an enzyme involved in unusual amino acid metabolism suggests the interesting possibility that mammalian mu-crystallins could be enzymes participating in processes such as osmoregulation or the metabolism of excitatory amino acids.