Protein Synthesis in Isolated Mitochondria of Rice (Oryza sativa L.) Seedlings.
Protein Synthesis in Isolated Mitochondria of Rice (Oryza sativa L.) Seedlings.
复制标题
水稻（Oryza sativa L.）幼苗分离线粒体中的蛋白质合成。
DOI：
10.1104/pp.96.1.319
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发表时间：
1991
期刊：
影响因子：
7.4
通讯作者：
K. Chiang
中科院分区：
文献类型：
--
作者：
H. Dai;Y. Lo;C. Wu;C. Tsou;G. Hsu;C. Chern;M. Ruddat;K. Chiang
为了研究水稻线粒体蛋白质的合成，常规的表面消毒程序被证明是无效的。由于污染细菌对[（35）S]甲硫氨酸的过度有效利用，即使是“基本无菌”的水稻线粒体也不适合用于细胞器蛋白质合成的研究。我们开发了一个程序，以获得无菌制备的水稻线粒体。这种线粒体有利于膜依赖的ATP生成系统，而不是外部ATP生成系统，作为细胞器蛋白质合成的能量补充。两个不同的类[（35）S]甲硫氨酸标记，环己酰亚胺不敏感的产品进行检测：一个电泳未解决的人口和一组约22至27个离散的多肽种类，每个具有特征的电泳迁移率和相对丰度。
For studies of in organello mitochondrial protein synthesis in rice, Oryza sativa L., conventional surface-sterilization procedures were demonstrated to be ineffective. Because of the over-whelmingly efficient [(35)S]methionine utilization by contaminating bacteria, even "essentially bacteria-free" rice mitochondria were shown to be unsuitable for the study of in organello protein synthesis. We developed a procedure to obtain a bacteria-free preparation of rice mitochondria. Such mitochondria favored a membrane-dependent ATP-generating system over an external ATP-generating system as the energy supplement for in organello protein synthesis. Two distinct classes of [(35)S]methionine-labeled, cycloheximide-insensitive products were detected: an electrophoretically unresolved population and a set of some 22 to 27 discrete polypeptide species, each with a characteristic electrophoretic mobility and relative abundance.