Extracellular matrix fibronectin alters connexin43 expression by alveolar epithelial cells
Extracellular matrix fibronectin alters connexin43 expression by alveolar epithelial cells
复制标题
DOI：
10.1152/ajplung.2001.280.4.l680
复制
发表时间：
2001-04-01
影响因子：
4.9
通讯作者：
Rannels, DE
中科院分区：
文献类型：
--
作者：
Alford, AI;Rannels, DE
肺泡II型上皮细胞经历表型变化，并建立间隙连接细胞间通讯，因为他们达到融合的原代培养。间隙连接蛋白（connexin）表达的模式平行变化。虽然连接蛋白（Cx）43的mRNA和蛋白质的培养第2天显着增加，Cx 26和Cx 32的表达下降。沿着Cx43表达的增加，细胞将来源于培养基中的血清和来源于从头合成的纤连蛋白组装到细胞外基质（ECM）中。目前的研究表明，这种ECM调节Cx43的表达。在含有8-10%胎牛血清（FBS）的DMEM中培养II型细胞促进富含纤连蛋白的ECM的组装，所述ECM刺激Cx43 mRNA和蛋白的表达。虽然Cx43蛋白的表达增加，以剂量依赖性的方式，在FBS的情况下，纤连蛋白也升高Cx43蛋白。抗纤连蛋白抗体显着降低血清依赖性的Cx43表达的增加。这些结果支持的前提是，ECM中的纤连蛋白有助于在原代培养的肺泡上皮细胞的Cx43表达的调节。
Alveolar type II epithelial cells undergo phenotypic changes and establish gap junction intercellular communication as they reach confluence in primary culture. The pattern of gap junction protein (connexin) expression changes in parallel. Although connexin (Cx)43 mRNA and protein increase significantly by culture day 2, Cx26 and Cx32 expression decline. Along with increasing Cx43 expression, the cells assemble fibronectin derived both from serum in the culture medium and from de novo synthesis into the extracellular matrix (ECM). The present studies indicate that this ECM regulates Cx43 expression. Culture of type II cells in DMEM containing 8-10% fetal bovine serum (FBS) promotes assembly of a fibronectin-rich ECM that stimulates expression of both Cx43 mRNA and protein. Although Cx43 protein expression increased in response to FBS in a dose-dependent manner, fibronectin also elevated Cx43 protein in the absence of FBS. Anti-fibronectin antibody significantly reduced the serum-dependent increase in Cx43 expression. These results support the premise that fibronectin in the ECM contributes to the regulation of Cx43 expression by alveolar epithelial cells in primary culture.