Susceptibility testing: accurate and reproducible minimum inhibitory concentration (MIC) and non-inhibitory concentration (NIC) values
Susceptibility testing: accurate and reproducible minimum inhibitory concentration (MIC) and non-inhibitory concentration (NIC) values
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DOI：
10.1046/j.1365-2672.2000.01017.x
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发表时间：
2000-05-01
影响因子：
4
通讯作者：
Pearson, J
中科院分区：
文献类型：
--
作者：
Lambert, RJW;Pearson, J
用现有方法测量一种物质的最小抑菌浓度（MIC）是简单直接的，然而从这些测试中获取有用的比较信息可能会更困难。本文报道了一种简单的技术和一种数据分析方法，能让实验人员从药敏试验中获得更多有用信息。这种方法使用一块100孔的微量滴定板，分析过程利用了通过比浊法从微量滴定板的每一个孔中获得的所有生长信息。采用一种改良的龚珀兹函数来拟合数据，由此可以得到更精确的MIC值。该技术还表明，在抑制剂的某些浓度下，相对于对照孔（无抑制剂），对生长没有影响。在一个阈值（被称为非抑制浓度或NIC）之上，生长受到限制，直至达到MIC，此时观察到相对于对照没有生长。
Measuring the minimum inhibitory concentration (MIC) of a substance by current methods is straightforward, whereas obtaining useful comparative information from the tests can be more difficult. A simple technique and a method of data anal! sis are reported which give the experimentalist moro useful information from susceptibility testing. This method makes use of a 100-well microtitre plate and the analysis uses all the growth information, obtained by turbidometry, from each and every well of the microtitre plate. A modified Gompertz function is used to fit the data, from which a more exact value can be obtained for the MIC. The technique also showed that at certain concentrations of inhibitor, there was no effect on growth relative to a control well (zero inhibitor). Above a threshold value, which has been termed the non-inhibitory concentration or NIC, growth becomes limiting until it reaches the MIC, where no growth relative to the control is observed.