ELIMINATION OF NONSPECIFIC ADSORPTION OF SERUM-PROTEINS BY SEPHAROSE-BOUND ANTIGENS
ELIMINATION OF NONSPECIFIC ADSORPTION OF SERUM-PROTEINS BY SEPHAROSE-BOUND ANTIGENS
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DOI：
10.1016/0003-2697(78)90679-6
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发表时间：
1978-01-01
影响因子：
2.9
通讯作者：
LEACH, SJ
中科院分区：
文献类型：
--
作者：
SMITH, JA;HURRELL, JGR;LEACH, SJ
血清蛋白的非特异性吸附发生在抗体对琼脂糖-肌红蛋白和琼脂糖-葡萄球菌核酸酶免疫吸附剂的免疫吸附上。这种吸附是由于这些血清蛋白和免疫吸附剂之间的非特异性疏水和离子相互作用引起的。对各种洗脱前洗涤程序进行了检查，只有含有非离子去污剂吐温20（0.1%）和高盐浓度（1 m NaCl）的硼酸盐-盐水缓冲液（pH 8.5）消除或显著减少了非特异性吸附，而没有明显减少特异性吸附抗体的回收。
Nonspecific adsorption of serum proteins occurs with immunoadsorption of antibodies on Sepharose-myoglobin and Sepharose-staphylococcal nuclease immunoadsorbents. This adsorption results from nonspecific hydrophobic and ionic interactions between these serum proteins and the immunoadsorbents. Various preelution washing procedures were examined, and only borate-saline buffer (pH 8,5) containing a nonionic detergent, Tween 20 (0,1%), and a high salt concentration (1 m NaCl) eliminated or significantly reduced nonspecific adsorption without appreciably diminishing the recovery of specifically adsorbed antibodies.