Multi‐Material Scaffolds for Tissue Engineering
Multi‐Material Scaffolds for Tissue Engineering
复制标题
用于组织工程的多材料支架
DOI：
10.1002/masy.200550935
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Wei Sun
中科院分区：
文献类型：
--
作者：
A. Darling;L. Shor;S. Khalil;M. Mondrinos;P. Lelkes;S. Güçeri;Wei Sun
为组织工程开发生物相容支架的一个趋势是寻找一种理想的单一材料，对其特定的细胞类型将表现出良好的行为。虽然一种理想的单一材料已经被证明是难以捉摸的，但使用特殊材料的组合制造支架可以产生更多功能的结构。通过控制材料成分的百分比和结构，可以针对给定的功能优化机械性能、细胞附着和增殖。将三种特殊材料，聚己内酯(PCL)、纤维蛋白和海藻酸盐结合到多组分支架中，进行了一系列实验，以测试每一组分与成纤维细胞的培养。坚硬和可成形的PCL提供了结构，纤维蛋白孔填充物允许细胞附着，海藻酸线提供了代替血管系统的营养转移途径。在培养7-12天后，根据成纤维细胞的分布和数量来判断支架的效果。
A trend in developing biocompatible scaffolds for tissue engineering has been to seek an ideal single material for which a given cell type will exhibit favorable behavior. While an ideal single material has proven elusive, scaffold manufacture using combinations of specialist materials can produce more versatile structures. By controlling the percentage and architecture of material components, mechanical properties, cell attachment, and proliferation may be optimized for a given function. Three specialist materials, poly-e-caprolactone (PCL), fibrin, and alginate, were incorporated into multi-component scaffolds for a series of experiments testing each component with culture of fibroblasts. The rigid and formable PCL provided structure, the fibrin pore-filler allowed for cell attachment, and alginate thread provided a nutrient transfer pathway in lieu of a vascular system. The efficacy of these scaffolds was judged on fibroblast distribution and population after 7-12 days of culture.