The respiratory burst of phagocytic cells: facts and problems.
The respiratory burst of phagocytic cells: facts and problems.
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吞噬细胞的呼吸爆发：事实和问题。
DOI：
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发表时间：
1982
影响因子：
--
通讯作者：
R. Basford
中科院分区：
文献类型：
--
作者：
Filippo Rossi;P. Bellavite;Giorgio Berton;P. Dri;G. Zabucchi;R. Basford
1.所谓的“可溶性”氧化酶不参与豚鼠和人粒细胞以及豚鼠腹膜驻留和引发的巨噬细胞的呼吸爆发。2.膜结合NAD（P）H氧化酶对NADPH氧化的激活是吞噬细胞呼吸激活的主要机制。3.氧化酶在静息细胞中是无活性的，活化形式在质膜上起作用。4.体外无细胞颗粒氧化NAD（P）H的机制不止一种。这些机制因测定条件（pH值和底物浓度）而异。5.在体外最佳条件下，NADPH的酶促氧化实际上涉及氧还原的单价途径，其中对于氧化的1纳摩尔NADPH形成2纳摩尔的O2的化学计量。6.同样在完整细胞中，所有O2首先被单向还原为O2，然后被排出细胞外或进入吞噬空泡。7.在完整细胞中参与O2平衡的主要反应是O2的单价还原、O2歧化成H2 O2以及通过过氧化氢和过氧化氢机制降解过氧化物。8.根据H_2O_2降解的机理，由活化的氧化酶单次还原的总氧是细胞消耗的净氧的2-4倍。9.所有的外呼吸速率都是由膜结合酶氧化生理浓度的NADPH的速率来解释的。这种充分性只有在适当的实验条件下才能观察到，因为氧化酶的高活性并不是永久状态。
1. The so called "soluble" oxidase(s) are not involved in the respiratory burst of guinea pig and human granulocytes and of guinea pig peritoneal resident and elicited macrophages. 2. The activation of the oxidation of NADPH by a membrane bound NAD(P)H oxidase is the main mechanism responsible for the activation of the respiration of phagocytes. 3. The oxidase is inactive in resting cells and the activated form works on the plasma membrane. 4. More than one mechanism is operative in the oxidation of NAD(P)H by cell free particles in vitro. These mechanisms vary in relation to the conditions of assay (pH and concentration of substrate). 5. Under optimal conditions in vitro the enzymatic oxidation of NADPH practically involves the univalent pathway of oxygen reduction with stoichiometry of two nanomoles of O2 formed for one nanomole of NADPH oxidized. 6. Also in intact cells all O2 is first univalently reduced to O2 and then discharged outside the cell or in the phagocytic vacuoles. 7. The main reactions involved in the O2 balance in intact cells are the univalent reduction of O2, the dismutation of O2 to H2O2 and the degradation of the peroxide through catalatic and peroxidatic mechanisms. 8. The total oxygen univalently reduced by the activated oxidase is 2-4 folds the net oxygen consumed by the cells, depending on the mechanism of H2O2 degradation. 9. All the rate of extrarespiration is accounted for by the rate of oxidation of physiological concentration of NADPH by the membrane-bound enzyme. This adequacy can be observed only under appropriate experimental conditions, because the high activity of the oxidase is not a permanent state.
慢性肉芽肿病——细胞和分子难题的一部分。
影响因子：
7.4
作者：
Babior,BM;Curnutte,JT
通讯作者：
Curnutte,JT