RNA‐seq based transcriptional analysis reveals dynamic genes expression profiles and immune‐associated regulation under heat stress in Apostichopus japonicus
RNA‐seq based transcriptional analysis reveals dynamic genes expression profiles and immune‐associated regulation under heat stress in Apostichopus japonicus
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DOI：
10.1016/j.fsi.2018.04.037
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发表时间：
2018-07
影响因子：
4.7
通讯作者：
Dongxue Xu;Shun Zhou;Lina Sun
中科院分区：
文献类型：
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作者：
Dongxue Xu;Shun Zhou;Lina Sun
本研究利用RNA-seq技术研究了持续热胁迫（6 h、48 h和192 h）下仿刺参（Apostichopus japonicus）的基因表达谱。在3个热胁迫处理组中分别检测到676、1010和1083个差异表达基因，表明不同的生物学过程有着复杂的调控。然后重点研究了高温胁迫下海参免疫系统的变化。热应激反应涉及的免疫相关基因主要有热休克蛋白、转铁蛋白超家族成员、效应基因、蛋白酶、补体系统、模式识别受体和信号转导等。通过真实的PCR检测免疫相关基因的mRNA表达。我们的研究结果表明，在这个物种的免疫策略，以应对突然升高的温度在环境中。
In this study, we explored the gene expression profiles inApostichopus japonicusunder continuous heat stress (6 h, 48 h and 192 h) by applying RNA-seq technique. A total of 676, 1010 and 1083 differentially expressed genes were detected at three heat stress groups respectively, which suggested complex regulation of various biological processes. Then we focused on the changing of immune system under HS in sea cucumbers. Key immune-associated genes were involved in heat stress response, which were classified into six groups: heat shock proteins, transferrin superfamily members, effector genes, proteases, complement system, and pattern recognition receptors and signaling. Moreover, the mRNA expression of the immune-associated genes were validated by the real time PCR. Our results showed that an immunological strategy in this species was developed to confront abrupt elevated temperatures in the environment.