The BRO proteins of Bombyx mori nucleopolyhedrovirus are nucleocytoplasmic shuttling proteins that utilize the CRM1-mediated nuclear export pathway
The BRO proteins of Bombyx mori nucleopolyhedrovirus are nucleocytoplasmic shuttling proteins that utilize the CRM1-mediated nuclear export pathway
复制标题
DOI：
10.1016/j.virol.2006.01.008
复制
发表时间：
2006-06-20
期刊：
影响因子：
3.7
通讯作者：
Matsumoto, Shogo
中科院分区：
文献类型：
--
作者：
Kang, WonKyung;Kurihara, Masaaki;Matsumoto, Shogo
家蚕核型多角体病毒（BmNPV）的BRO蛋白在感染过程中表现出胞内定位的双相模式。在早期，它们位于细胞核中，但随着感染的进行，它们随后显示出细胞质和细胞核定位。因此，我们研究了核出口的可能性。使用抑制剂，我们发现BmNPV BRO蛋白穿梭于细胞核和细胞质之间。BRO蛋白富含亮氨酸区域的突变导致瞬时表达的蛋白质在核中积累，这表明该区域起着CRM 1依赖的核输出信号（内斯）的作用。相反，具有改变的内斯的突变体BRO-D在感染的细胞中不显示核积累，尽管蛋白质产生似乎减少。RT-PCR分析表明，较低水平的蛋白质产量是由于RNA合成的减少。两者合计，我们的研究结果表明，BRO蛋白是利用CRM 1介导的核输出途径的核质穿梭蛋白。(c)2006年爱思唯尔公司All rights reserved.
The BRO proteins of Bombyx mori nucleopolyhedrovirus (BmNPV) display a biphasic pattern of intracellular localization during infection. At early times, they reside in the nucleus but then show both cytoplasmic and nuclear localization as the infection proceeds. Therefore, we examined the possibility of nuclear export. Using inhibitors, we reveal that BmNPV BRO proteins shuttle between the nucleus and cytoplasm. Mutations on the leucine-rich region of BRO proteins resulted in nuclear accumulation of transiently expressed proteins, suggesting that this region functions as a CRM1-dependent nuclear export signal (NES). On the contrary, mutant BRO-D with an altered NES did not show nuclear accumulation in infected cells, although protein production seemed to be reduced. RT-PCR analysis showed that the lower level of protein production was due to a reduction in RNA synthesis. Taken together, our results suggest that BRO proteins are nucleocytoplasmic shuttling proteins that utilize the CRM1-mediated nuclear export pathway. (c) 2006 Elsevier Inc. All rights reserved.