IMMUNOHISTOCHEMICAL LOCALIZATION OF RAT ANGIOTENSIN-II AT1 RECEPTOR
IMMUNOHISTOCHEMICAL LOCALIZATION OF RAT ANGIOTENSIN-II AT1 RECEPTOR
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DOI：
10.1152/ajprenal.1993.264.6.f989
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发表时间：
1993-06-01
影响因子：
--
通讯作者：
BERNSTEIN, KE
中科院分区：
文献类型：
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作者：
PAXTON, WG;RUNGE, M;BERNSTEIN, KE
为了研究血管紧张素II的受体，制备了针对对应于大鼠AT 1A和AT 1B受体的氨基酸15-24的肽QDDCPKAGRHC的多克隆兔抗肽抗血清。大鼠组织的Western分析显示约43 kDa的主要条带。抗血清免疫沉淀AT 1受体蛋白在体外产生。大鼠组织的免疫组织化学分析显示动脉和小动脉平滑肌的强染色。其他含有AT 1受体蛋白的组织包括肝细胞、肾上腺的肾小球、支气管、肠、输尿管和附睾的平滑肌。在肾脏中，在所有小动脉和小动脉中观察到强染色。传入和传出小动脉含有近似相等强度的免疫反应性AT 1蛋白。外髓质的内条在厚的升肢上皮内有中等水平的受体。近端肾小管上皮细胞也表达受体蛋白。肾小球免疫反应性AT 1蛋白存在于肾小球系膜细胞内，并且在不同的大鼠品系中强度不同。刘易斯和Wistar大鼠表现出中度肾小球染色，而CD和Sprague-Dawley品系表现出较低水平的反应性。肾小球系膜细胞含有AT 1蛋白的事实支持了系膜细胞是血管紧张素II在肾小球内作用的主要场所的概念。
To study receptors for angiotensin II, polyclonal rabbit anti-peptide antisera were prepared against the peptide QDDCPKAGRHC corresponding to amino acids 15-24 of the rat AT1A and AT1B receptors. Western analysis of rat tissues showed a major band of approximately 43 kDa. The antisera immunoprecipitated AT1-receptor protein produced in vitro. Immunohistochemical analysis of rat tissues showed intense staining of arterial and arteriolar smooth muscle. Other tissues that contained AT1-receptor protein included hepatocytes, the zona glomerulosa of the adrenal gland, and the smooth muscle of the bronchus, gut, ureter, and epididymis. In the kidney, intense staining was observed in all small arteries and arterioles. Both afferent and efferent arterioles contain approximately equal intensities of immunoreactive AT1 protein. The inner stripe of the outer medulla has a moderate level of receptors within thick ascending limb epithelium. Proximal tubular epithelium also expresses receptor protein. Glomerular immunoreactive AT1 protein is found within mesangial cells and varies in intensity among different rat strains. Lewis and Wistar rats demonstrated moderate glomerular staining, whereas the CD and Sprague-Dawley strains showed lesser levels of reactivity. The fact that glomerular mesangial cells contain AT1 protein supports the concept that mesangial cells are the primary locus of angiotensin II action within the glomerulus.