Enhancing nisin yield by engineering a small noncodding RNA anti41 and inhibiting the expression of glnR in Lactococcus lactis F44
Enhancing nisin yield by engineering a small noncodding RNA anti41 and inhibiting the expression of glnR in Lactococcus lactis F44
复制标题
通过改造小型非编码 RNA anti41 并抑制乳酸乳球菌 F44 中 glnR 的表达来提高乳链菌肽产量
DOI：
10.1007/s10529-018-2550-3
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发表时间：
2018-06-01
影响因子：
2.7
通讯作者：
Qiao, Jianjun
中科院分区：
文献类型：
--
作者：
Miao, Sen;Wu, Hao;Qiao, Jianjun
目的：设计一个小的非编码RNA anti 41，通过抑制乳酸乳球菌F44中GlnR的表达来提高乳链菌肽的产量。结果：构建了筛选合适的人工sRNA的文库，获得了一个对GlnR的抑制效果约为3倍的sRNA anti 41。此外，在抗41过表达菌株（F44-抗41）中，GlnR的直接抑制性靶点（glnD、glnQ、amtB和glnK）的转录水平显著上调，从而证实了抗41对GlnR的抑制作用。此外，anti 41基因的过表达可使细胞在酸胁迫下的存活率提高约3倍，并促进细胞生长，使乳链菌肽产量提高29.83%。
Objectives:To engineer a small nonconding RNA anti41 to enhance nisin yield by inhibiting the expression of glnR in Lactococcus lactis F44.Results:We constructed a screening library to determine appropriate artificial sRNAs and obtained a sRNA anti41 that can produce approximately three fold of the inhibitory effect on GlnR. Moreover, the transcription levels of the direct inhibitory targets of GlnR (glnP, glnQ, amtB, and glnK) were dramatically upregulated in the anti41 overexpression strain (F44-anti41), thereby confirming the inhibitory effect of anti41 on GlnR. In addition, anti41 overexpression improved the survival rate of cells by approximately three fold under acid stress, promoted cell growth, and increased nisin yield by 29.83%.Conclusions:We were able to provide a novel strategy for the construction of robust high-producing industrial strains.