Polymorphism of Red Cell NADP-Dependent Isocitrate Dehydrogenase in Macaque Monkeys
Polymorphism of Red Cell NADP-Dependent Isocitrate Dehydrogenase in Macaque Monkeys
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猕猴红细胞 NADP 依赖性异柠檬酸脱氢酶的多态性
DOI：
10.1537/ase1911.82.52
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发表时间：
1974
期刊：
影响因子：
--
通讯作者：
T. Shotake
中科院分区：
文献类型：
--
作者：
G. Ishimoto;M. Kuwata;T. Shotake
总共对 1704 只猕猴进行了 NADP 依赖性异柠檬酸脱氢酶电泳变体的筛查，并在来自 9 种猕猴不同种群的血液样本中发现了该酶的 6 种表型。据透露，来自中国的穆拉塔 (M. mulatta) 具有最多的多态性，三个假设的等位基因导致六种表型。 M. cyclopis 和 M. irus（菲律宾组除外）具有两个具有三种表型的等位基因，其余物种对于两个常见等位基因（Idhmac1 和 Idhmac2）之一是纯合的。其中一个有趣的发现是，根据等位基因分布，猕猴物种可以分为两类：具有高频率 Idhmac2 的物种（M. fuscata、M. spiosa、M. mulatta 和 M. cyclopis）和具有高频率 Idhmac1 的物种（M. irus、M. nemestrina、M. radiata 和 M. niger）。这可能表明两组猕猴之间在系统发育上的不同位置。 NADP 依赖性异柠檬酸脱氢酶（IDH；EC 1.1.1.42）催化 L-异柠檬酸氧化脱羧为 α-酮戊二酸，在大多数动物中以两种形式存在：一种位于线粒体部分的细胞中，另一种存在于可溶性部分中（PLAUT，1963）。至于IDH的可溶形式，已在多种动物中证实了遗传变异。在人类中，大多数个体通过电泳显示出单个 IDH 条带，但迄今为止已经发现了三种变体，每种变体都具有三个均匀间隔的酶带，并具有特征性的迁移率，在近 2000 个不同种族血统的血液样本中，总频率低于 0.2%（CHEN 等，1972）。一般来说，这样的三带模式被认为代表了二聚体蛋白中的典型杂合子，并且在其他物种的可溶性IDH杂合子中也报道了相同类型的模式，例如小鼠（HENDERSON，1965）和果蝇（Fox，1971），其遗传被确定为常染色体共显性。本研究关注猕猴可溶性 IDH 的电泳变化。我们发现某些种类的猕猴中出现电泳变异的频率如此之高* 这项研究部分得到了教育部的资助。猕猴中的多态性 53 个普遍认为是多态性的。尽管家族研究无法以猴子为对象，但我们推测猕猴的 IDH 也是由单个位点决定的，因为猕猴酶的电泳模式由一个或三个条带组成。本文讨论了由九种猕猴组成的不同地理种群中表型分布的种间和种内差异。
A total of 1704 macaques were screened for electrophoretic variants of NADP-dependent isocitrate dehydrogenase and six phenotypes of the enzyme were found in the blood samples from various populations of nine macaque species. M. mulatta from China was disclosed to be most polymorphic, with three postulated alleles resulting in six phenotypes. M. cyclopis and M. irus (except the philippine group) had two alleles with three phenotypes and the remaining species were homozygous for one of the two common alleles (Idhmac1 and Idhmac2). One of the interesting finding was that the macaque species can be divided into two groups on the basis of the allelic distribution: those species with a high frequency of the Idhmac2 (M. fuscata, M. speciosa, M. mulatta, and M. cyclopis) and those with a high frequency of the Idhmac1 (M. irus, M. nemestrina, M. radiata, and M. niger). This might suggest phylogenetically divergent position between two groups of the macaque species. NADP-dependent isocitrate dehydrogenase (IDH; EC 1.1.1.42), which catalyzes the oxidative decarboxylation of L-isocitrate to a-ketoglutarate, is present in two forms in most animals: one is located in cells in the mitochondrial fraction and the other is found in the soluble fraction (PLAUT, 1963). As to the soluble form of IDH, genetic variation has been demonstrated io several species of animals. In man, most individuals show a single band of the IDH by electrophoresis, but three variants, each of which has three evenly spaced enzyme bands with characteristic mobilites, have so far been found, giving an overall frequency of less than 0.2% in nearly 2000 blood specimens of various ethnic origins (CHEN et al., 1972). In general, such triple band patterns are considered to represent the typical heterozygotes in a dimeric protein, and the same kind of pattern has been reported in heterozygotes for soluble IDH in other species, such as mouse (HENDERSON, 1965) and drosophila (Fox, 1971), of which inheritance isascertained to be autosomal codominant. The present study is concerned with the electrophoretic variation in the soluble IDH of macaque monkeys. We found that electrophoretic variants occur in certain species of macaques with such frequen* This study was in part supported by the grants from the Ministry of Education. Polymorphism in macaque monkeys 53 cies that generally accepted as to be polymorphic. Although the family studies were unable to perform monkey subjects , we supposed that the IDH in macaques is also determined by a single locus since the pattern of macaque enzyme consists of either one or three bands on electrophoresis. This paper deals with the inter and intra-species differences in the distribution of the phenotypes among various geographic populations comprising nine species of macaques.