Vertical transmission of bovine leukemia virus and bovine immunodeficiency virus in dairy cattle herds
Vertical transmission of bovine leukemia virus and bovine immunodeficiency virus in dairy cattle herds
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DOI：
10.1016/s0378-1135(01)00458-8
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发表时间：
2002-01-23
影响因子：
3.3
通讯作者：
Onuma, M
中科院分区：
文献类型：
--
作者：
Meas, S;Usui, T;Onuma, M
在北海道的 5 个奶牛群中调查了牛白血病病毒 (BLV) 和牛免疫缺陷病毒 (BIV) 的垂直传播，其中 36.1% 和 17.0% 的牛分别为 BLV 和 BIN 血清阳性，9.9% 的母牛同时感染 BIV 和 BLV。对 26 例仅感染 BLV（17 例）或同时感染 BIN 和 BLV（9 例）的母鼠所生的后代，通过聚合酶链反应（PCR）和合胞体测定在初乳喂养前后检查 BLV 和 BIV 的存在。出生后，所有小牛都立即与母牛分开。 BLV 阳性母鼠所生的后代在初乳喂养前 BLV 呈阴性，表明未发生经胎盘传播。此后，这些后代被喂食母牛的初乳或牛奶，但 BLV 仍然呈阴性。 BLV 阳性母鼠出生的其他后代则用 BLV 阴性初乳或巴氏灭菌的 BLV 阳性初乳喂养。所有这些犊牛的 BLV 感染均呈阴性，表明 BLV 的子宫内传播可以忽略不计。 在 BLV 和 BIV 共同感染的母牛所生的后代中，犊牛在出生后 1 天的初乳喂养前呈 BIV 阳性，表明 BIV 的子宫内传播。在母牛喂养初乳后，新生牛犊呈 BLV 阳性。此外，一只小牛甚至在初乳喂养之前就呈 BLV 阳性。这些结果表明 BIV 可以在子宫内传播给后代。如果母鼠同时感染 BIV 和 BLV，则 BLV 可以通过初乳或牛奶传播。 (C) 2002 Elsevier Science B.V. 保留所有权利。
Vertical transmission of bovine leukemia virus (BLV) and bovine immunodeficiency virus (BIV) was investigated in five dairy cattle herds in Hokkaido, where 36.1 and 17.0% of cattle were BLV and BIN seropositive, respectively, and 9.9% of dams were co-infected with both BIV and BLV. Twenty six cases of offspring born from dams infected with only BLV (17 cases) or with both BIN and BLV (9 cases) were examined for the presence of BLV and BIV before and after colostrum feeding by polymerase chain reaction (PCR) and syncytium assay. After birth, all calves were separated immediately from their dams. The offspring born from BLV-positive dams were BLV-negative before colostrum feeding, suggesting that no transplacental transmission had occurred. Thereafter, these offspring were fed colostrum or milk from their dams, but still remained BLV-negative. The other offspring born from BLV-positive dams were fed with BLV-negative colostrum, or with pasteurized BLV-positive colostrum. All these calves remained negative for BLV infection, suggesting that in utero transmission of BLV is negligible.In the case of offspring born from darns co-infected with BLV and BIV, calves were BIV-positive before colostrum feeding at I day after the birth, indicating in utero transmission of BIV. After colostrum feeding from their dams, newborn calves became BLV-positive. In addition, one calf was BLV-positive even before colostrum feeding. These results suggest that BIV can be transmitted to offspring in utero. and that BLV can be transmitted through colostrum or milk if dams are infected with both BIV and BLV. (C) 2002 Elsevier Science B.V. All rights reserved.