The First Report on Fin Rot Disease of Cultured Turbot Scophthalmus maximus in China
The First Report on Fin Rot Disease of Cultured Turbot Scophthalmus maximus in China
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DOI：
10.1577/h04-050.1
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发表时间：
2006-06
影响因子：
1.2
通讯作者：
Q. Lei;Wang Yin-geng;Zhang Zheng;Yang Shao-li
中科院分区：
文献类型：
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作者：
Q. Lei;Wang Yin-geng;Zhang Zheng;Yang Shao-li
摘要自1998年以来，大菱鲆养殖在我国北方沿海沿着迅速发展，成为一项重要的产业。然而，在2001年至2004年的流行病学研究中发现了烂鳍病。感染的死亡率很高，在某些情况下高达90-100%。幼鱼对感染很敏感。一个重要的临床症状是鳍腐烂。在四个案例中进行了细菌分离，包括孵化场和农场。经形态学、生理生化试验和16 SrRNA序列分析，确定优势菌株为鳗弧菌（Vibrioanguillarum），其直径为0.2-0.4 × 1.5-2.0 μm。用分离的鳗弧菌进行的毒力测定导致与自然感染的鱼相同的大体症状，表明鳗弧菌是烂鳍病的病原体。对50%鱼的14天致死剂量计算为1.7 × 103菌落形成单位/mL。组织学研究表明鳗弧菌能引起严重的组织改变。
Abstract Since 1998, the culture of turbot Scophthalmus maximus has spread rapidly along the coast of North China, becoming an important industry. However, fin rot disease was identified during an epidemiological study from 2001 to 2004. The infections occurred with high mortality, up to 90–100% in some cases. Young fish are sensitive to the infection. An important clinical sign is fin rot. Bacterial isolation was done in four cases, including hatcheries and farms. One dominant strain of 0.2–0.4 × 1.5–2.0 μm was identified as Vibrio anguillarum by morphology, physiological and biochemical tests, and 16S rRNA sequence analysis. Virulence assays carried out with the isolated V. anguillarum resulted in the same gross signs as in naturally infected fish, indicating that V. anguillarum was the causative agent for fin rot disease. The 14-d dose lethal to 50% of the fish was calculated as 1.7 × 103 colony-forming units/mL. Histopathological studies demonstrated that V. anguillarum could cause severe tissue chang...