miR-203 Expression in Exfoliated Cells of Tongue Coating Represents a Sensitive and Specific Biomarker of Gastroesophageal Reflux Disease.
miR-203 Expression in Exfoliated Cells of Tongue Coating Represents a Sensitive and Specific Biomarker of Gastroesophageal Reflux Disease.
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舌苔脱落细胞中 miR-203 的表达是胃食管反流病的敏感且特异的生物标志物
DOI：
10.1155/2016/2349453
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发表时间：
2016
影响因子：
2
通讯作者：
Zhang H
中科院分区：
文献类型：
--
作者：
Yan X;Zhu S;Zhang H
背景和目的。MicroRNAs(MiRNAs)参与了包括胃食道反流病(GERD)在内的多种人类疾病的病理生理过程。本研究的目的是研究GERD患者和健康对照组(CtrlS)舌脱落细胞miRNA的表达。方法：研究方法。应用定量逆转录聚合酶链式反应(qRT-PCR)检测GERD(n=24)和CtrlS(n=24)6个候选miRNAs(miR-143、miR-145、miR-192、miR-194、miR-203和miR-205)的表达水平。这些发现在验证队列中得到了证实(GERD，n=142；CtrlS，n=48)。用Mann-Whitney U检验评价miRNA表达水平的差异，用受试者-操作者特征(ROC)曲线获得特异性和敏感性。结果。与Ctrls(P<；0.0001)相比，MIR-203在GERD患者中的表达显著下调，其ROC曲线为0.94(95%CI：0.9-0.97)。MiR-203对GERD和CtrlS的敏感性和特异性分别为91.7%和87.3%。提示miR-203可能是鉴别GERD和CtrlS的有用的诊断标志物。结论。MIR-203检测可能有助于有GERD症状的患者的诊断。
Background and Aim. MicroRNAs (miRNAs) have been implicated in the pathophysiology of numerous human diseases including gastroesophageal reflux disease (GERD). The objective of this study was to investigate the miRNA expression of exfoliated cells of the tongue in patients with GERD versus healthy controls (Ctrls). Methods. Using quantitative reverse-transcription PCR (qRT-PCR), expression levels of six candidate miRNAs (miR-143, miR-145, miR-192, miR-194, miR-203, and miR-205) were examined across a discovery cohort of patients with GERD (n = 24) versus Ctrls (n = 24). These findings were confirmed across a validation cohort (GERD, n = 142; Ctrls, n = 48). Differences in miRNA expression levels were evaluated using the Mann-Whitney U test while the specificity and sensitivity were obtained using receiver-operator characteristic (ROC) curves. Results. miR-203 was significantly downregulated in GERD patients as compared to Ctrls (P < 0.0001) with ROC curve of 0.94 (95% CI: 0.90–0.97). The sensitivity and the specificity of miR-203 were 91.7% and 87.3%, respectively, in the GERD and Ctrls. These results suggest that miR-203 may be a useful diagnostic marker for discriminating GERD from Ctrls. Conclusions. miR-203 testing may assist in the diagnosis of patients with symptoms suggestive of GERD.
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影响因子：
2.6
作者：
Bus, P.;Siersema, P. D.;van Baal, J. W. P. M.
通讯作者：
van Baal, J. W. P. M.
幽门螺杆菌感染诱导的miR-203下调通过靶向CASK促进胃癌的增殖和侵袭。
影响因子：
--
作者：
Zhou X;Xu G;Yin C;Jin W;Zhang G
通讯作者：
Zhang G
影响因子：
--
作者：
Zhu, Xiangyu;Er, Kejun;Shi, Hong
通讯作者：
Shi, Hong
影响因子：
9.6
作者：
Wijnhoven, B. P. L.;Hussey, D. J.;Michael, M. Z.
通讯作者：
Michael, M. Z.
miR-638是一种新的生物标志物，用于预测接受化疗的非小细胞肺癌患者的预后。
影响因子：
12.8
作者：
Wang, Fang;Lou, Jian-fang;Cao, Yan;Shi, Xin-hui;Wang, Peng;Xu, Jian;Xie, Er-fu;Xu, Ting;Sun, Rui-hong;Rao, Jian-yu;Huang, Pu-wen;Pan, Shi-yang;Wang, Hong
通讯作者：
Wang, Hong