Response of Heat-Shock Protein (HSP) Genes to Temperature and Salinity Stress in the Antarctic Psychrotrophic Bacterium Psychrobacter sp G
Response of Heat-Shock Protein (HSP) Genes to Temperature and Salinity Stress in the Antarctic Psychrotrophic Bacterium Psychrobacter sp G
复制标题
南极耐冷细菌 Psychrobacter sp. 热休克蛋白 (HSP) 基因对温度和盐度胁迫的响应。
DOI：
10.1007/s00284-013-0409-3
复制
发表时间：
2013-11-01
影响因子：
2.6
通讯作者：
Lin, Xuezheng
中科院分区：
文献类型：
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作者：
Che, Shuai;Song, Weizhi;Lin, Xuezheng
温度和盐度的波动是影响极地细菌生长的两个最重要的因素。为了更好地了解热休克蛋白（heat-shock proteins，HSPs）在南极嗜冷菌Psychrobacter sp. G适应性机制中的作用，在基因组草图的基础上克隆了Hsp 845、Hsp 2538、Hsp 2666和Hsp 2667基因。采用qRT-PCR方法分析了不同胁迫条件下HSP基因的表达特征。Hsp 845和Hsp 2667的表达被低温（分别为0和10 °C）显著抑制。0 °C时，Hsp 2538和Hsp 2666的表达无明显差异，而10 °C时，Hsp 2666的表达受到抑制。Hsp 2538和Hsp 2667的表达不敏感，但Hsp 845和Hsp 2666的表达在低盐度（分别为0和15）下增加。4个HSP基因的表达在高盐度（90和120）和高温下的盐度无关的增强。相比之下，低温没有显着的影响独立的盐度。
Temperature and salinity fluctuations are two of the most important factors affecting the growth of polar bacteria. In an attempt to better understand the function of heat-shock proteins (HSPs) in the adaptive mechanisms of the Antarctic psychrotrophic bacterium Psychrobacter sp. G to such conditions, genes Hsp845, Hsp2538, Hsp2666, and Hsp2667 were cloned on the basis of the draft genome. The expression characteristics of these HSP genes under different stress conditions were analyzed by the qRT-PCR method. Expression of Hsp845 and Hsp2667 was inhibited significantly by low temperature (0 and 10 °C, respectively). There was no difference of expression when Hsp2538 and Hsp2666 were exposed to 0 °C but the expression of Hsp2666 was inhibited when exposed to 10 °C. Expression of Hsp2538 and Hsp2667 was not sensitive but expression of Hsp845 and Hsp2666 was increased at low salinity (0 and 15, respectively). Expression of the four HSP genes was enhanced at high salinity (90 and 120) and at high temperature independent of salinity. By contrast, low temperature had no significant effect independent of salinity.