MicroRNA-638 inhibits human airway smooth muscle cell proliferation and migration through targeting cyclin D1 and NOR1.
MicroRNA-638 inhibits human airway smooth muscle cell proliferation and migration through targeting cyclin D1 and NOR1.
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DOI：
10.1002/jcp.26930
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发表时间：
2018-01
影响因子：
5.6
通讯作者：
Sun J
中科院分区：
文献类型：
--
作者：
Wang H;Yao H;Yi B;Kazama K;Liu Y;Deshpande D;Zhang J;Sun J
Abnormal airway smooth muscle cell (ASMC) proliferation and migration contribute significantly to increased ASM mass associated with asthma. MicroRNA (miR)-638 is a primate-specific miRNA that plays important roles in development, DNA damage repair, hematopoiesis, and tumorigenesis. Although it is highly expressed in ASMCs, its function in ASM remodeling remains unknown. In the present study, we found that in response to various mitogenic stimuli, including Platelet-derived growth factor (PDGF)-BB, Transforming Growth Factor Beta 1 (TGF-β1), and Fetal Bovine Serum (FBS), the expression of miR-638, as determined by quantitative real-time polymerase chain reaction (qRT-PCR), was significantly downregulated in the proliferative human ASMCs. Both gain- and loss-of-function studies were performed to study the role of miR-638 in ASMC proliferation and migration. We found that adenovirus-mediated miR-638 overexpression markedly inhibits ASMC proliferation and migration, while ablation of miR-638 by anti-miR-638 markedly increases cell proliferation and migration, as determined by WST-8 proliferation and scratch wound assays. Dual-luciferase reporter assay, qRT-PCR, and immunoblot analysis were used to investigate the effects of miR-638 on the expression of the downstream target genes in ASMCs. Our results demonstrated that miR-638 overexpression significantly reduced the expression of downstream target cyclin D1 and NOR1, both of which have been shown to be essential for cell proliferation and migration. Together, our study provides the first in vitro evidence highlighting the anti-proliferative and anti-migratory roles of miR-638 in human ASMC remodeling and suggests that targeted overexpression of miR-638 in ASMCs may provide a novel therapeutic strategy for preventing ASM hyperplasia associated with asthma.
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影响因子：
3.6
作者：
Cheng, Wei;Yan, Kun;Dang, Cheng-Xue
通讯作者：
Dang, Cheng-Xue
MicroRNA-638 在人血管平滑肌细胞中高表达，通过靶向孤儿核受体 NOR1 抑制 PDGF-BB 诱导的细胞增殖和迁移
影响因子：
10.8
作者：
Li, Pan;Liu, Yan;Sun, Jianxin
通讯作者：
Sun, Jianxin
影响因子：
12.3
作者：
Christenson SA;Brandsma CA;Campbell JD;Knight DA;Pechkovsky DV;Hogg JC;Timens W;Postma DS;Lenburg M;Spira A
通讯作者：
Spira A
DOI：
10.1046/j.1440-1681.2001.03426.x
发表时间：
2001-03-01
影响因子：
2.9
作者：
Johnson, PRA
通讯作者：
Johnson, PRA
影响因子：
3.8
作者：
Deshpande, D. A.;Dileepan, M.;Kannan, M. S
通讯作者：
Kannan, M. S