The regulation of oxidative phosphorylation pathway on Vibrio alginolyticus adhesion under adversities
The regulation of oxidative phosphorylation pathway on Vibrio alginolyticus adhesion under adversities
复制标题
逆境下氧化磷酸化途径对溶藻弧菌粘附的调控
DOI：
10.1002/mbo3.805
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发表时间：
2019-08-01
期刊：
影响因子：
3.4
通讯作者：
Yan, Qingpi
中科院分区：
文献类型：
--
作者：
Huan, Li;Huang, Lixing;Yan, Qingpi
溶藻弧菌是海水养殖中最重要的病原体之一，导致严重损失。经过 Cu2+、Pb2+ 和低 pH 处理后，RNA-seq 和定量实时 PCR 发现氧化磷酸化途径基因（包括 coxA、coxB、coxC、ccoN、ccoO 和 ccoQ）的表达普遍下调。 RNAi 显着降低溶藻弧菌中 coxA、coxB、coxC、ccoN、ccoO 和 ccoQ 的表达。与野生型菌株相比，溶藻弧菌RNAi菌株的粘附能力以及细胞色素C氧化酶活性均显着受损。 ccoQ在这些靶基因中对细菌粘附的调节似乎更重要，而ccoO对粘附的调节相对较弱。同时，温度、盐度、pH和饥饿的变化对coxA、coxB、coxC、ccoN、ccoO和ccoQ的表达影响显着。这些发现表明：氧化磷酸化途径是溶藻弧菌粘附的关键调节因子； coxA、coxB、coxC、ccoN、ccoO 和 ccoQ 调节细菌粘附以响应温度、盐度、pH 和饥饿等环境变化。
Vibrio alginolyticus is one of the most important pathogens in mariculture and leading to heavy losses. After treatment with Cu2+, Pb2+, and low pH, the expression of oxidative phosphorylation pathway genes, including coxA, coxB, coxC, ccoN, ccoO, and ccoQ, was found commonly downregulated by RNA‐seq as well as quantitative real‐time PCR. RNAi significantly reduced the expression of coxA, coxB, coxC, ccoN, ccoO, and ccoQ in V. alginolyticus. Compared with the wild‐type strain, the adhesion abilities of RNAi strains of V. alginolyticus were significantly impaired, as well as their cytochrome C oxidase activity. ccoQ appeared to be more important in the regulation of bacterial adhesion in these target genes, while ccoO was relatively weak in the regulation of the adhesion. Meanwhile, the changes of temperature, salinity, pH, and starvation affected coxA, coxB, coxC, ccoN, ccoO, and ccoQ expression remarkably. These findings indicated that: the oxidative phosphorylation pathway is a critical regulator of adhesion in V. alginolyticus; coxA, coxB, coxC, ccoN, ccoO, and ccoQ regulate the bacterial adhesion in response to environmental changes such as temperature, salinity, pH, and starvation.