RELEASE OF MONOAMINES FROM STRIATUM OF RAT AND MOUSE EVOKED BY LOCAL APPLICATION OF POTASSIUM - EVALUATION OF A NEW INVIVO ELECTROCHEMICAL TECHNIQUE
RELEASE OF MONOAMINES FROM STRIATUM OF RAT AND MOUSE EVOKED BY LOCAL APPLICATION OF POTASSIUM - EVALUATION OF A NEW INVIVO ELECTROCHEMICAL TECHNIQUE
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DOI：
10.1111/j.1471-4159.1986.tb13048.x
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发表时间：
1986-03-01
影响因子：
4.7
通讯作者：
HOFFER, BJ
中科院分区：
文献类型：
--
作者：
GERHARDT, GA;ROSE, GM;HOFFER, BJ
通过微压喷射局部施加K+，结合体内电化学检测，研究麻醉大鼠和小鼠纹状体单胺神经末梢的刺激释放。K+诱发的释放是可逆的、可重复的和剂量依赖性的。相反，电活性物质的释放不能通过局部喷射Na+来引起。小鼠尾状核记录的K+诱发释放量和时间过程均大于大鼠。局部应用诺米芬，一种假定的儿茶酚胺再摄取阻滞剂，增加了K+诱发释放的幅度和时间过程。纹状体附近的大脑区域也记录到了释放；这些信号总是比尾状核中看到的信号小，其振幅与这些区域的多巴胺能输入的相对程度显示出良好的对应关系。这些数据与文献中的其他信息一起表明，这种新技术非常适合于完整动物单胺释放和再摄取的原位研究。
Local application of K+ via micropressure-ejection, coupled with in vivo electrochemical detection, was used to study stimulated release from monoaminergic nerve termials in the striatum of anesthetized rats and mice. K+-evoked releases were reversible, reproducible, and dose-dependent. In contrast, releases of electroactive species could not be evoked by local ejection of Na+. The magnitudes and time courses of K+-evoked releases recorded from the caudate nucleus of mice were greater than those seen in rats. Local application of nomifensine, a putative catecholamine reuptake blocker, augmented the magnitudes and time courses of K+-evoked releases. Releases were also recorded from brain regions adjacent the striatum; these signals were always smaller than those seen in the caudate nucleus and had amplitudes that showed good correspondence to the relative degree of dopaminergic input to these areas. These data, taken together with other information in the literature, suggest that this new technique is well suited for in situ studies of monoamine release and reuptake in intact animals.