Interaction of peanut agglutinin with normal human lymphocytes and with leukemic cells.
Interaction of peanut agglutinin with normal human lymphocytes and with leukemic cells.
复制标题
花生凝集素与正常人淋巴细胞和白血病细胞的相互作用。
DOI：
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发表时间：
1979
影响因子：
11.1
通讯作者：
B. Ramot
中科院分区：
文献类型：
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作者：
Y. Reisner;M. Biniaminov;E. Rosenthal;N. Sharon;B. Ramot
用异硫氰酸荧光素标记花生凝集素（PNA），研究了PNA与人胸腺细胞、外周血淋巴细胞和不同类型白血病患者外周血细胞的相互作用。大多数人胸腺细胞（60-80%）结合凝集素。胸腺细胞的主要亚群，是PNA阳性的分离PNA阴性细胞的差异凝集与凝集素。用混合淋巴细胞反应和菜豆植物血凝素对两种胸腺细胞亚群进行了检测。PNA阳性胸腺细胞对这些刺激的反应较差，表明这些胸腺细胞在功能上是不成熟的。白血病患者外周血淋巴细胞的荧光素异硫氰酸酯-PNA结合试验表明，在大多数急性白血病中，PNA受体暴露在母细胞上，而在大多数慢性白血病的情况下，外周血淋巴细胞是PNA阴性的。PNA作为未成熟血细胞标记物的有效性及其潜在的临床应用进行了讨论。
The interaction of peanut agglutinin (PNA) with human thymocytes, peripheral blood lymphocytes, and peripheral blood cells of various types of leukemia was investigated by using fluorescein isothiocyanate-conjugated PNA. The majority of human thymocytes (60-80%) bind the lectin. The major subpopulation of thymocytes that is PNA-positive was separated from the PNA-negative cells by differential agglutination with the lectin. The two thymocyte subpopulations were tested in the mixed lymphocyte reaction and with the phytohemagglutinin of Phaseolus vulgaris. The poor response of the PNA-positive thymocytes to these stimuli indicates that these thymocytes are functionally immature. The fluorescein isothiocyanate-PNA-binding test with peripheral blood lymphocytes of leukemic patients revealed that in most acute leukemias the PNA receptor is exposed on the blastic cells, whereas in most cases of chronic leukemia the peripheral blood lymphocytes are PNA-negative. The validity of PNA as a marker of immature blood cells and its potential clinical application are discussed.