Targeted Lipidomics of Mycobacterial Lipids and Glycolipids
Targeted Lipidomics of Mycobacterial Lipids and Glycolipids
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DOI：
10.1007/978-1-0716-1460-0_24
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发表时间：
2021-01-01
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影响因子：
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通讯作者：
Layre, Emilie
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作者：
Layre, Emilie
数十年的研究强调了分枝杆菌产生的脂质和糖脂家族的丰富性和独特性。其中许多家族以刺激或抑制的方式有效调节宿主免疫反应。因此，在不同遗传背景或感染模型条件下对这一库的全球研究正在引起人们的兴趣。尽管存在与该库的特异性相关的困难，但基于质谱的分枝杆菌脂质组学领域最近取得了相当大的进展，特别是在分析水平上。进一步取得进展的空间仍然很大，特别是在开发用于分析生成的大型数据集的有效生物信息学管道方面。本章介绍了一种 HPLC-MS 方法，可同时筛选分枝杆菌产生的 20 多种脂质家族，并提供了分析现有技术所生成数据的建议。
Decades of study have highlighted the richness and uniqueness of the repertoire of lipid and glycolipid families produced by mycobacteria. Many of these families potently regulate host immune responses, in stimulatory or suppressive ways. Thus, the global study of this repertoire in different genetic backgrounds or under model conditions of infection is gaining interest. Despite the difficulties associated with the specificities of this repertoire, the field of mass spectrometry-based lipidomics of mycobacteria has recently made considerable progress, particularly at the analytical level. There is still considerable scope for further progress, especially with regard to the development of an efficient bioinfomatics pipeline for the analysis of the large datasets generated. This chapter describes an HPLC-MS methodology allowing the simultaneous screening of more than 20 of the lipid families produced by mycobacteria and provides recommendations to analyze the generated data given the state-of-the-art.