Optimal depth and duration of mild hypothermia in a focal model of transient cerebral ischemia - Effects on neurologic outcome, infarct size, apoptosis, and inflammation
Optimal depth and duration of mild hypothermia in a focal model of transient cerebral ischemia - Effects on neurologic outcome, infarct size, apoptosis, and inflammation
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DOI：
10.1161/01.str.29.10.2171
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发表时间：
1998-10-01
期刊：
影响因子：
8.3
通讯作者：
Steinberg, GK
中科院分区：
文献类型：
--
作者：
Maier, CM;Ahern, KV;Steinberg, GK
背景和目的-亚低温可能是迄今为止发展的唯一最有效的脑保护方法。然而，许多关于亚低温的问题仍有待解决，在其潜在的实施在人类中风的治疗。在这里，我们报告的结果，旨在确定最佳的深度和持续时间的局部脑卒中和其对梗死面积，神经系统的结果，程序性细胞死亡，和inflammation. Methods大鼠进行了2小时闭塞的左大脑中动脉的研究。在第一项研究（I）中，将动物保持（局部缺血）在37 ℃（n=8）、33 ℃（n= 8）或30 ℃（n=8）。研究II包括4组：（1）对照组（37 ℃，n=10），（2）缺血发作时开始30分钟低温（33 ℃，n=9），（3）1小时（33 ℃，n=8），（4）2小时（33 ℃，n=8）。通过放置在对侧皮质中的热电偶探针测量脑温度。拆线后，所有动物复温并再灌注22小时（I）或70小时（II）。结果-相对于对照组（53 +/-8%，P
Background and Purpose-Mild hypothermia is possibly the single most effective method of cerebroprotection developed to date. However, many questions regarding mild hypothermia remain to be addressed before its potential implementation in the treatment of human stroke. Here we report the results of 2 studies designed to determine the optimal depth and duration of mild hypothermia in focal stroke and its effects on infarct size, neurological outcome, programmed cell death, and inflammation.Methods-Rats underwent a 2-hour occlusion of the left middle cerebral artery. In the first study (I) animals were kept (intraischemically) at either 37 degrees C (n=8), 33 degrees C (n= 8), or 30 degrees C (n=8). Study II consisted of 4 groups: (1) controls (37 degrees C, n=10), (2) 30 minutes of hypothermia started at ischemic onset (33 degrees C, n=9), (3)1 hour (33 degrees C, n=8), and (4) 2 hours (33 degrees C, n=8). Brain temperature was measured by a thermocouple probe placed in the contralateral cortex. After suture removal, all animals were rewarmed and reperfused for 22 hours (I) or 70 hours (II).Results-Mild hypothermia to 33 degrees C or 30 degrees C was neuroprotective (17+/-7% and 27+/-6%, respectively) relative to controls (53 +/- 8%, P