Human neutrophil response to short-term exposure to F-75 cobalt-based alloy.
Human neutrophil response to short-term exposure to F-75 cobalt-based alloy.
复制标题
人类中性粒细胞对短期接触 F-75 钴基合金的反应。
DOI：
10.1002/jbm.820260911
复制
发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Black,J
中科院分区：
文献类型：
--
作者：
Yang,J;Shanbhag,A;Lilien,J;Black,J
研究了生物材料对人中性粒细胞活化的影响。人中性粒细胞与F-75钴基合金或聚苯乙烯微球孵育，微球的大小为不可吞噬的大小，中性粒细胞的平面总面积是F-75的两倍。采用扫描电子显微镜、透射电子显微镜、能量色散X射线微区分析、石墨炉原子吸收光谱分析等手段，分析了样品中金属含量和细胞形态的变化。有证据表明，人PMN在外来物质刺激下表现出形态变化，包括F-75珠面的活化，腐蚀产物的胞吞作用，细胞内空泡的形成，脱颗粒等。此外，当PMN存在时，F-75的腐蚀释放速率比无细胞对照增加了三倍。©1992 John Wiley父子公司
The effect of biomaterials on the activation of human neutrophils was studied. Human neutrophils were incubated with F‐75 cobalt‐based alloy or polystyrene microspheres of a nonphagocytosable size with two times total neutrophil plane surface area. Scanning and transmission electron microscope (SEM, TEM), energy dispersive x‐ray microanalysis (EDX), and graphite furnace atomic absorption spectroscopy (GFAAS) were used to analyze changes in cellular morphology and metal content. This report presents evidence that human PMNs display morphological changes related to foreign material challenge, including activation on F‐75 bead surfaces, pinocytosis of corrosion products, formation of intracellular vacuoles, degranulation, etc. Moreover, when PMNs were present, the corrosion release rate of F‐75 increased as much as three times over cell‐free controls. © 1992 John Wiley & Sons, Inc.