RALF4/19 peptides interact with LRX proteins to control pollen tube growth in Arabidopsis
RALF4/19 peptides interact with LRX proteins to control pollen tube growth in Arabidopsis
复制标题
DOI：
10.1126/science.aao5467
复制
发表时间：
2017-12-22
期刊：
影响因子：
56.9
通讯作者：
Grossniklaus, Ueli
中科院分区：
文献类型：
--
作者：
Mecchia, Martin A.;Santos-Fernandez, Gorka;Grossniklaus, Ueli
细胞外基质的变化与细胞内部的沟通对于细胞的功能至关重要。快速碱化因子（RALF）家族的细胞外肽已被鉴定为CrRLK1L亚类受体样激酶的配体，但其感知的确切机制尚不清楚。我们发现，拟南芥RALF4和RALF19冗余地调节花粉管的完整性和生长，并且它们的功能依赖于花粉表达的富含亮氨酸重复延伸蛋白（LRX）家族的蛋白质，该家族在细胞壁发育中发挥作用，但其作用方式尚不清楚。LRX蛋白与RALF相互作用，监测细胞壁的变化，这些变化通过CrRLK1L途径传递到花粉管内部以维持正常生长。
The communication of changes in the extracellular matrix to the interior of the cell is crucial for a cell's function. The extracellular peptides of the RAPID ALKALINIZATION FACTOR (RALF) family have been identified as ligands of receptor-like kinases of the CrRLK1L subclass, but the exact mechanism of their perception is unclear. We found that Arabidopsis RALF4 and RALF19 redundantly regulate pollen tube integrity and growth, and that their function depends on pollen-expressed proteins of the LEUCINE-RICH REPEAT EXTENSIN (LRX) family, which play a role in cell wall development but whose mode of action is not understood. The LRX proteins interact with RALFs, monitoring cell wall changes, which are communicated to the interior of the pollen tube via the CrRLK1L pathway to sustain normal growth.