Cytochemical and cytofluorometric evidence for guard cell photosystems.
Cytochemical and cytofluorometric evidence for guard cell photosystems.
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保卫细胞光系统的细胞化学和细胞荧光证据。
DOI：
10.1104/pp.71.2.420
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发表时间：
1983
期刊：
影响因子：
7.4
通讯作者：
W. H. Outlaw
中科院分区：
文献类型：
--
作者：
K. Vaughn;W. H. Outlaw
用两种高空间分辨率的灵敏方法获得了保卫细胞光合线性电子传递的证据。在大麦保卫细胞质体中观察到光依赖的二氨基联苯胺氧化（PSI的指示剂）和DCMU敏感的光依赖的硫代氨基甲酰硝基蓝四唑还原（PSII的指示剂）。cv Himalaya的电镜细胞化学方法。在蚕豆保卫细胞对中检测到DCMU敏感的Chl a荧光诱导（PSII的指示剂）。cv Longpod的荧光光谱分析。至少对于这些物种，我们得出结论，这些结果是PSII的保卫细胞叶绿体，这到目前为止一直有些争议的存在的证据。
Evidence for photosynthetic linear electron transport in guard cells was obtained with two sensitive methods of high spacial resolution. Light-dependent diaminobenzidine oxidation (an indicator of PSI) and DCMU-sensitive, light-dependent thiocarbamyl nitroblue tetrazolium reduction (an indicator of PSII) were observed in guard cell plastids of Hordeum vulgare L. cv Himalaya using electron microscopic cytochemical procedures. DCMU-sensitive Chl a fluorescence induction (an indicator of PSII) was detected in individual guard cell pairs of Vicia faba L. cv Longpod using an ultramicrofluorometer. At least for these species, we conclude these results are proof for the presence of PSII in guard cell chloroplasts, which until now has been somewhat controversial.