COLLOIDAL GOLD AS A BIOCOMPATIBLE IMMOBILIZATION MATRIX SUITABLE FOR THE FABRICATION OF ENZYME ELECTRODES BY ELECTRODEPOSITION
COLLOIDAL GOLD AS A BIOCOMPATIBLE IMMOBILIZATION MATRIX SUITABLE FOR THE FABRICATION OF ENZYME ELECTRODES BY ELECTRODEPOSITION
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DOI：
10.1002/bit.260400406
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发表时间：
1992-08-05
影响因子：
3.8
通讯作者：
HENKENS, RW
中科院分区：
文献类型：
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作者：
CRUMBLISS, AL;PERINE, SC;HENKENS, RW
葡萄糖氧化酶、辣根过氧化物酶、黄嘌呤氧化酶和碳酸酐酶已被吸附到胶体金溶胶上，并具有良好的酶活性保留。黄嘌呤氧化酶在胶体金上的吸附不会导致酶活性的变化，这是通过用化学计量抑制剂蝶呤醛进行活性位点滴定和通过测量表观米氏常数（K（M ′））来确定的。金溶胶吸附葡萄糖氧化酶，辣根过氧化物酶，黄嘌呤氧化酶也被电沉积到导电矩阵（铂纱布和/或玻璃碳），使酶电极。这些电极保留了酶的活性，更重要的是，在适当的电子转移介体的存在下，给出了对酶底物的电化学响应。我们的研究结果表明，实用的胶体金作为一种生物相容性的酶固定化基质，适用于制造酶电极。
Glucose oxidase, horseradish peroxidase, xanthine oxidase, and carbonic anhydrase have been adsorbed to colloidal gold sols with good retention of enzymatic activity. Adsorption of xanthine oxidase on colloidal gold did not result in a change in enzymatic activity as determined by active site titration with the stoichiometric inhibitor pterin aldehyde and by measurement of the apparent Michaelis constant (K(M)'). Gold sols with adsorbed glucose oxidase, horseradish peroxidase, and xanthine oxidase have also been electro-deposited onto conducting matrices (platinum gauze and/or glassy carbon) to make enzyme electrodes. These electrodes retained enzymatic activity and, more importantly, gave an electrochemical response to the enzyme substrate in the presence of an appropriate electron transfer mediator. Our results demonstrate the utility of colloidal gold as a biocompatible enzyme immobilization matrix suitable for the fabrication of enzyme electrodes.