Profiles of NK, NKT cell activation and cytokine production following vaccination against hepatitis B
Profiles of NK, NKT cell activation and cytokine production following vaccination against hepatitis B
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DOI：
10.1111/j.1600-0463.2005.apm_191.x
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发表时间：
2005-07-01
期刊：
影响因子：
2.8
通讯作者：
Berrueta, L
中科院分区：
文献类型：
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作者：
Albarran, B;Goncalves, L;Berrueta, L
人类自然杀伤 (NK) 细胞 (CD56+ CD3-) 是先天免疫系统的重要组成部分，特别是针对病毒感染，因为它们的激活可以调节适应性免疫反应的结果。 NKT 细胞 (CD56+CD3+) 是一种以表达 NK 细胞表面标志物为特征的淋巴细胞 T 群体，已知在肝脏中含量丰富，其激活可能与肝损伤有关。使用三色流式细胞术测量表面受体和细胞内细胞因子，我们探索了一组接种过乙型肝炎疫苗和未接种疫苗的个体中 NK 和 NKT 细胞的早期激活信号和细胞因子的产生。在疫苗接种反应者中与重组 HBsAg 一起孵育后，在 NK 细胞中观察到 CD56（亮）细胞群、激活受体 CD69 和 IFN-γ 的特异性增加。在 NKT 细胞中观察到了类似的结果，显示应答受试者中 CD69、CD25、IL-2 和 IFN-γ 表达增加。在两组细胞中无反应个体中，这些参数在统计上均有所减少（p < 0.05）。这些结果表明，在对疫苗无反应的人中，这些细胞的活化减弱，表明 NK 和 NKT 细胞在乙型肝炎疫苗接种后的免疫反应中发挥着重要作用。
Human natural killer (NK) cells (CD56+ CD3-) represent crucial components of the innate immune system especially against viral infections and because their activation can modulate the outcome of the adaptive immune response. NKT cells (CD56+CD3+), a lymphocyte T population characterized by expression of surface markers of NK cells, are known to be abundant in the liver and their activation could be associated with hepatic injury. Using three-color flow cytometry to measure surface receptors and intracellular cytokines, we have explored early activation signals and cytokine production in NK and NKT cells within a group of hepatitis B vaccinated and non-vaccinated individuals. A specific increase of the CD56(bright) cell population, the activation receptor CD69 and IFN-gamma, was observed in NK cells following incubation with recombinant HBsAg in responders to vaccination. Comparable results were observed in NKT cells showing an increment of CD69, CD25, IL-2 and IFN-gamma expression in responder subjects. These parameters were statistically diminished in non-responder individuals (p < 0.05) in both groups of cells. These results demonstrate a diminished activation of these cells in non-responders to the vaccine, suggesting that NK and NKT cells play an important role in the immune response following hepatitis B vaccination.