The photodynamic effect of tetra-substituted N-methyl-pyridyl-porphine combined with the action of vancomycin or host defense mechanisms disrupts Staphylococcus epidermidis biofilms
The photodynamic effect of tetra-substituted N-methyl-pyridyl-porphine combined with the action of vancomycin or host defense mechanisms disrupts Staphylococcus epidermidis biofilms
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DOI：
10.1177/039139880903200906
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发表时间：
2009-09-01
影响因子：
1.7
通讯作者：
Visai, Livia
中科院分区：
文献类型：
--
作者：
Sbarra, Maria S.;Arciola, Carla R.;Visai, Livia
表皮葡萄球菌是引起医院感染的重要病原体。毒力可归因于生物膜的形成，生物膜提供了保护细菌免受宿主免疫系统和化疗攻击的微环境。在本研究中，我们扩展到S。epidermidis策略以前旨在治疗S.金黄色葡萄球菌生物膜的光动力学治疗（PDT）结合化疗或吞噬作用。当结构上不同的生物膜暴露于阳离子卟啉，四取代的N-甲基-吡啶基-卟啉（TMP），并同时暴露于可见光时，观察到细菌存活的显着减少。值得注意的是，生物膜清除的程度取决于其成熟阶段：发育中的年轻生物膜比成熟生物膜对PDT更敏感。此外，PDT处理的生物膜暴露于万古霉素或全血的吞噬作用几乎完全根除。我们获得的数据表明，PDT与抗生素或宿主防御相结合也可能是灭活S.表皮生物膜(Int J Artiff Organs 2009; 32：574-83）
The skin commensal and opportunistic pathogen Staphylococcus epidermidis is an important cause of nosocomial infections. Virulence is attributable to formation of biofilm, which provides a microenvironment that protects the bacterium from attack by the host immune system and by chemotherapy. In this study we extended to S. epidermidis strategies previously aimed at treatment of S. aureus biofilms using photodynamic treatment (PDT) combined with chemotherapy or phagocytosis. A significant reduction in bacterial survival was observed when structurally distinct biofilms were exposed to the cationic porphyrin, tetra-substituted N-methyl-pyridyl-porphine (TMP), and simultaneously to visible light. Of note, the extent of biofilm clearance depended on its maturation stage: developing, young biofilms, were more sensitive towards PDT than mature biofilms. Furthermore, PDT-treated biofilms exposed to vancomycin or subjected to phagocytic action of whole blood were almost completely eradicated. The data we obtained establish that PDT combined with antibiotics or host defenses may also be a useful approach for the inactivation of S. epidermidis biofilms. (Int J Artif Organs 2009; 32: 574-83)