Possible role of preproghrelin gene polymorphisms in susceptibility to bulimia nervosa
Possible role of preproghrelin gene polymorphisms in susceptibility to bulimia nervosa
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DOI：
10.1002/ajmg.b.30387
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发表时间：
2006-12-05
影响因子：
2.8
通讯作者：
Ichimaru, Yuhei
中科院分区：
文献类型：
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作者：
Ando, Tetsuya;Komaki, Gen;Ichimaru, Yuhei
先前的研究表明，生长素释放肽（ghrelin）是一种内源性食欲肽，与饮食失调的病理学有关。我们进行了一项研究，以确定前饥饿素原基因多态性是否与饮食失调有关。 336 名饮食失调患者，包括 131 名神经性厌食症 (AN) 限制型 (AN-R)、97 名 AN 暴食/清除型 (AN-BP) 和 108 名神经性贪食症 (BN) 清除型 (BN-P) 以及 300 名健康对照受试者参与了这项研究。进行基因分型以确定存在的多态性，并利用该信息分析标记之间的连锁不平衡（LD），并比较各组之间的基因型分布、等位基因频率和单倍型频率。外显子 2 中的 Leu72Met (408 C > A) (rs696217) 多态性和内含子 2 中的 3056 T > C (rs2075356) 多态性处于 LD (D' = 0.902，r(2) = 0.454)。两种多态性均与 BN-P 显着相关（等位基因方面：P = 0.0410，比值比 (OR) = 1.48；对于 Leu72Met 和 3056 T > C，分别为 P = 0.0035，OR = 1.63）。此外，我们观察到 BN-P 患者中单倍型 Met72-3056C 的频率显着增加（P = 0.0059，OR = 1.71）。我们的研究结果表明，前胃饥饿素基因的 Leu72Met (408 C > A) 和 3056 T > C 多态性与 BN-P 的易感性相关。 (c) 2006 Wiley-Liss, Inc.
Previous investigations have suggested that ghrelin, an endogenous orexigenic peptide, is involved in the pathology of eating disorders. We conducted a study to determine whether any preproghrelin gene polymorphisms are associated with eating disorders. Three hundred thirty-six eating disorder patients, including 131 anorexia nervosa (AN)-restricting types (AN-R), 97 AN-binge eating/purging types (AN-BP) and 108 bulimia nervosa (BN)-purging types (BN-P), and 300 healthy control subjects participated in the study. Genotyping was performed to determine the polymorphisms present, and with this information, linkage disequilibrium (LD) between the markers was analyzed and the distributions of the genotypes, the allele frequencies, and the haplotype frequencies were compared between the groups. The Leu72Met (408 C > A) (rs696217) polymorphism in exon 2 and the 3056 T > C (rs2075356) Volymorphism in intron 2 were in LD (D' = 0.902, r(2) = 0.454). Both polymorphisms were significantly associated with BN-P (allele-wise: P = 0.0410, odds ratio (OR) = 1.48; P = 0.0035, OR = 1.63, for Leu72Met and 3056 T > C, respectively). In addition, we observed a significant increase in the frequency of the haplotype Met72-3056C in BN-P patients (P = 0.0059, OR = 1.71). Our findings suggest that the Leu72Met (408 C > A) and the 3056 T > C polymorphisms of the preproghrelin gene are associated with susceptibility to BN-P. (c) 2006 Wiley-Liss, Inc.