Effects of Curcumin-mediated photodynamic therapy on autophagy and epithelial-mesenchymal transition of lung cancer cells
Effects of Curcumin-mediated photodynamic therapy on autophagy and epithelial-mesenchymal transition of lung cancer cells
复制标题
姜黄素介导的光动力疗法对肺癌细胞自噬和上皮间质转化的影响
DOI：
10.1016/j.pdpdt.2022.102849
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发表时间：
2022-04-19
影响因子：
3.3
通讯作者：
Bai, Dingqun
中科院分区：
文献类型：
--
作者：
Shao, Lan;Zhu, Ying;Bai, Dingqun
背景：本研究旨在研究姜黄素介导的PDT是否可以抑制肺癌细胞中的EMT，并探索自噬在调节EMT的过程中的作用。方法：通过CCK-8测定评估肺癌细胞的活力。通过蛋白质印迹测定法测量了上皮标记和间充质标记，LC3-I向LC3-II的转化以及A549和SPCA1细胞中p62和Beclin1的水平。伤口愈合和Transwell分析用于检测A549和SPCA1细胞的迁移和侵袭能力。通过观察A549细胞中LAMP-2与LC3的共定位来检测到自噬体的形成，并通过TEM进行自噬超微结构观察：curcumin-PDT抑制EMT，迁移和侵袭，并在肺癌细胞中诱导自噬。姜黄素-PDT诱导的自噬参与了PDT抑制EMT的过程，但它在肺癌细胞中产生了EMT的促进作用。姜黄素-PDT与CQ结合进一步抑制EMT，肺癌细胞的侵袭和迁移。结论：PDT诱导的自噬在EMT调节中的作用被确定为促进肺癌的作用。因此，姜黄素介导的PDT与自噬抑制剂结合进一步抑制了肺癌细胞的EMT，并且可能代表了针对肺癌侵袭和迁移的潜在策略。
Background: This study aimed to investigated whether Curcumin-mediated PDT can suppress EMT in lung cancer cells, and explore the roles of autophagy in the process of regulating EMT.Methods: Lung cancer cell viability was assessed by CCK-8 assay. The expression of epithelial marker and mesenchymal markers, the conversion of LC3-I to LC3-II and the levels of p62 and beclin1 in A549 and SPCA1 cells were measured by Western blotting assay. The Wound healing and Transwell assays were used to detect the migration and invasion abilities of the A549 and SPCA1 cells. Autophagosome formation was detected via observing the colocalization of Lamp-2 with LC3 in A549 cells, and the autophagy ultrastructure was observed by TEM.Results: Curcumin-PDT inhibited EMT, migration and invasion and induced autophagy in lung cancer cells. Curcumin-PDT induced autophagy was involved in the process of PDT inhibiting EMT, but it presented a promoting effect of EMT in lung cancer cells. Curcumin-PDT combined with CQ further inhibited EMT, invasion and migration of lung cancer cells.Conclusions: The role of PDT-induced autophagy in the regulation of EMT was determined to be a promoting effect in lung cancer. Therefore, Curcumin-mediated PDT combined with autophagy inhibitor further suppressed EMT of lung cancer cells, and may represent a potential strategy against invasion and migration of lung cancer.