Diversity of Cervicovaginal Microbiota Associated with Female Lower Genital Tract Infections
Diversity of Cervicovaginal Microbiota Associated with Female Lower Genital Tract Infections
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DOI：
10.1007/s00248-011-9813-z
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发表时间：
2011-04-01
影响因子：
3.6
通讯作者：
Xiang, Charlie
中科院分区：
文献类型：
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作者：
Ling, Zongxin;Liu, Xia;Xiang, Charlie
女性生殖道（FGT）含有大量已知对阴道健康起重要作用的细菌物种。以前的研究集中在阴道中的细菌多样性，但对与FGT感染相关的宫颈外微生物群知之甚少。在我们的研究中，收集了100名中国受试者的阴道拭子和宫颈拭子，其中包括30名细菌性阴道病（BV; BV组），22名宫颈炎（Cer组），18名BV合并宫颈炎（BC组）和30名健康对照妇女（CN组）。采用非培养依赖性聚合酶链反应（PCR）-变性梯度凝胶电泳（DGGE）和定量PCR（qPCR）对11种与FGT感染相关的微生物进行了研究。尽管存在显著的人际差异，但PCR-DGGE图谱显示，BV组的阴道微生物群和宫颈外微生物群明显更为复杂，而宫颈外微生物群在健康和患病参与者之间没有显着差异。使用物种特异性qPCR，BV和宫颈炎与乳杆菌属物种的显著减少显著相关（p < 0.05），并且潜在致病物种如加德纳菌属、奇异菌属、埃格特菌属、Leptotrichia/Sneathia和普雷沃菌属在BV中比在CN样品中更常见且拷贝数更高（p值范围为0.000至0.021）。在健康和宫颈炎样品之间没有观察到宫颈外微生物群的显著差异（p > 0.05）。宫颈内细菌总数明显低于阴道内（p < 0.05）。有趣的是，BV合并宫颈炎的参与者的阴道微生物群与BV或宫颈炎单独参与者的阴道微生物群完全不同。我们的研究表明，宫颈阴道微生物群积极参与FGT感染的过程。宫颈阴道菌群的主要细菌明显与BV相关;然而，没有足够的证据表明宫颈外微生物群直接参与宫颈炎的发展。
The female genital tract (FGT) harbors very large numbers of bacterial species that are known to play an important role on vaginal health. Previous studies have focused on bacterial diversity in the vagina, but little is known about the ectocervical microbiota associated with FGT infections. In our study, vaginal swabs and ectocervical swabs were collected from 100 participants in China, including 30 women with bacterial vaginosis (BV; BV group), 22 women with cervicitis (Cer group), 18 women with BV in combination with cervicitis (BC group) and 30 healthy control women (CN group). The diversity and richness of cervicovaginal microbiota were investigated with culture-independent polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) and quantitative PCR (qPCR) targeting 11 microorganisms that have been associated with FGT infections. Despite significant interpersonal variations, the PCR-DGGE profiles revealed that vaginal microbiota and ectocervical microbiota were clearly much more complex in the BV group, while the ectocervical microbiota showed no significant difference between healthy and diseased participants. Using species-specific qPCR, BV and cervicitis were significantly associated with a dramatic decrease in Lactobacillus species (p < 0.05), and potential pathogenic species such as Gardnerella, Atopobium, Eggerthella, Leptotrichia/Sneathia, and Prevotella were more common and in higher copy numbers in BV than in CN samples (p values ranged from 0.000 to 0.021). No significant differences were observed between healthy and cervicitis samples (p > 0.05) in ectocervical microbiota. The total numbers of bacteria were significantly lower in the ectocervix as compared in the vagina (p < 0.05). Intriguingly, vaginal microbiota from participants with BV in combination with cervicitis was quite different from that of participants with BV or cervicitis alone. Our study demonstrated that the cervicovaginal microbiota was actively involved in the process of FGT infections. The predominant bacteria of the cervicovaginal communities were clearly associated with BV; however, there was not sufficient evidence that the ectocervical microbiota is directly involved in the development of cervicitis.