The characteristic expression of B7-associated proteins in Langerhans cell sarcoma
The characteristic expression of B7-associated proteins in Langerhans cell sarcoma
复制标题
B7 相关蛋白在朗格汉斯细胞肉瘤中的特征表达。
DOI：
10.1016/j.acthis.2011.12.010
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发表时间：
2012-01-01
影响因子：
2.5
通讯作者：
Chen, Yongwen
中科院分区：
文献类型：
--
作者：
Li, Hong;Wang, Changsong;Chen, Yongwen
郎格汉斯细胞肉瘤(LCS)是一种罕见的来源于表皮树突状细胞的恶性肿瘤，其特征是细胞学上的异型性、频繁的有丝分裂和侵袭性的临床行为。肿瘤相关的B7分子包括B7-H1、B7-DC、B7-H3和B7-H4，被认为参与了癌细胞的免疫逃逸，并作为预后标志物发挥作用。然而，这些分子在LCS中的表达和分布还没有被描述。在此，我们通过免疫组织化学分析，在LCS标本切片中观察到所有这些分子。在细胞水平上，它们存在于细胞膜和细胞质中。荧光双重染色显示B7-H1、B7-H3和B7-H4主要与Langerin(+)肿瘤细胞相关。更有趣的是，B7-H1、B7-H3和B7-H4在同一肿瘤细胞上共表达。新的B7相关蛋白Z39Ig也在LCS样本切片中被发现。荧光双重染色显示Z39Ig与CD68(+)巨噬细胞结合。我们的结果提示，B7-H1、B7-H3和B7-H4可能是识别LCS的潜在生物标志物，了解它们的功能可能会进一步阐明LCS的发病机制，并可能为开发新的免疫治疗策略做出贡献。(C)2012年爱思唯尔股份有限公司。版权所有。
Langerhans cell sarcoma (LCS) is a rare malignancy derived from dendritic cells of the epidermis that is characterized by cytological atypia, frequent mitoses, and aggressive clinical behavior. Cancer-associated B7 molecules including B7-H1, B7-DC, B7-H3 and B7-H4 are thought to be involved in the immunoescape of cancer cells and to function as prognostic markers. However, the expression and distribution of these molecules in LCS have not been described. Here we report that all of these molecules were observed in LCS sample sections by immunohistochemistry analysis. At the cellular level, they were found on the cell membrane and in the cytoplasm. Fluorescence dual staining indicated that B7-H1, B7-H3 and B7-H4 were principally associated with Langerin(+) tumor cells. More interestingly, B7-H1, B7-H3 and B7-H4 were co-expressed on the same tumor cells. Z39Ig, the novel B7-related protein, was also found in the LCS sample sections. Fluorescence dual staining showed that Z39Ig was restricted on CD68(+) macrophages. Our results suggest that B7-H1, B7-H3 and B7-H4 may be potential biomarkers to identify LCS, and a clear understanding of their functional roles may further elucidate the pathogenesis of this carcinoma and potentially contribute to the development of novel immunotherapeutic strategies. (c) 2012 Elsevier GmbH. All rights reserved.