DESIGN, CHEMICAL SYNTHESIS, AND EXPRESSION OF GENES FOR THE 3 HUMAN COLOR-VISION PIGMENTS
DESIGN, CHEMICAL SYNTHESIS, AND EXPRESSION OF GENES FOR THE 3 HUMAN COLOR-VISION PIGMENTS
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DOI：
10.1021/bi00112a002
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发表时间：
1991-12-03
期刊：
影响因子：
2.9
通讯作者：
PELLETIER, SL
中科院分区：
文献类型：
--
作者：
OPRIAN, DD;ASENJO, AB;PELLETIER, SL
人类的色觉是由视网膜视锥细胞的三种色素介导的：蓝色、绿色和红色。我们已经设计并化学合成了这三种色素的基因。基因在COS细胞中表达，用11-顺式-视网膜发色团重建，并使用免疫亲和程序纯化至均一。为了促进免疫亲和纯化，每个色素的羧基末端进行了修改，以包含一个额外的8个氨基酸的单克隆抗体先前用于纯化牛视紫红质的表位。分离的色素的光谱分别具有424、530和560 nm的最大值，对于蓝色、绿色和红色色素。这些最大值与先前通过显微分光光度法观察到的单个人视锥细胞的最大值非常一致。光谱是第一次从分离的人类色觉色素。他们证实了三个色觉基因的原始鉴定，这是基于遗传证据。
Color vision in humans is mediated by three pigments from retinal cone photoreceptor cells: blue, green, and red. We have designed and chemically synthesized genes for each of these three pigments. The genes were expressed in COS cells, reconstituted with 11-cis-retinal chromophore, and purified to homogeneity using an immunoaffinity procedure. To facilitate the immunoaffinity purification, each pigment was modified at the carboxy terminus to contain an additional eight amino acid epitope for a monoclonal antibody previously used to purify bovine rhodopsin. The spectra for the isolated pigments had maxima of 424, 530, and 560 nm, respectively, for the blue, green, and red pigments. These maxima are in excellent agreement with the maxima previously observed by microspectrophotometry of individual human cone cells. The spectra are the first to be obtained from isolated human color vision pigments. They confirm the original identification of the three color vision genes, which was based on genetic evidence.