BIOCHEMICAL ACTIVITIES OF THE PARA PARTITION PROTEIN OF THE P1 PLASMID
BIOCHEMICAL ACTIVITIES OF THE PARA PARTITION PROTEIN OF THE P1 PLASMID
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DOI：
10.1111/j.1365-2958.1992.tb01552.x
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发表时间：
1992-05-01
影响因子：
3.6
通讯作者：
AUSTIN, SJ
中科院分区：
文献类型：
--
作者：
DAVIS, MA;MARTIN, KA;AUSTIN, SJ
单位拷贝P1质粒的稳定性依赖于质粒编码的称为par的分隔区，由帕拉和parB基因以及parS位点组成。帕拉是划分所必需的，但它的划分关键作用尚不清楚。纯化的帕拉蛋白在体外显示出具有ATP酶活性，其被纯化的ParB蛋白和DNA特异性地刺激。帕拉负责调节帕拉和parB的表达，纯化的帕拉具有ATP依赖性、位点特异性DNA结合活性，其识别与帕拉启动子重叠的序列。的ATP依赖性的结合活性的作用，以及其他可能的功能的ATP酶活性的分区，进行了讨论。
The unit-copy P1 plasmid depends for stability on a plasmid-encoded partition region called par, consisting of the parA and parB genes and the parS site. ParA is absolutely required for partition, but its partition-critical role is not known. Purified ParA protein is shown to possess an ATPase activity in vitro which is specifically stimulated by purified ParB protein and by DNA. ParA is responsible for regulation of expression of parA and parB, and purified ParA has an ATP-dependent, site-specific DNA binding activity which recognizes a sequence that overlaps the parA promoter. The role of the ATP-dependence of the binding activity, as well as other possible functions of the ATPase activity in partition, is discussed.