Opposing roles for endogenous BDNF and NT-3 in regulating cortical dendritic growth
Opposing roles for endogenous BDNF and NT-3 in regulating cortical dendritic growth
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DOI：
10.1016/s0896-6273(00)80316-5
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发表时间：
1997-05-01
期刊：
影响因子：
16.2
通讯作者：
Lo, DC
中科院分区：
文献类型：
--
作者：
McAllister, AK;Katz, LC;Lo, DC
大脑皮层各层内的神经元表达神经营养因子家族的多个成员及其相应的受体。这种多样性可以提供功能冗余;或者，不同的神经营养因子可以指导皮质神经元生长和分化的不同方面。通过用Trk受体体（Trk-lgGs）中和发育中的皮层器官型切片中的内源性神经营养因子，我们发现BDNF和NT-3在调节锥体神经元的树突生长中彼此对立。在第4层，内源性和外源性NT-3均抑制BDNF刺激的树突生长。相反，在第6层中，内源性和外源性BDNF均抑制NT-3刺激的树突状细胞生长。内源性BDNF和NT-3的这些拮抗作用提供了一种机制，通过这种机制，树突状细胞的生长和收缩可以在皮质发育过程中动态调节，并表明在发育中的皮质中表达的多种神经营养因子代表了用于调节树突状细胞生长的细胞外信号系统的不同组分。
Neurons within each layer of cerebral cortex express multiple members of the neurotrophin family and their corresponding receptors. This multiplicity could provide functional redundancy; alternatively, different neurotrophins may direct distinct aspects of cortical neuronal growth and differentiation. By neutralizing endogenous neurotrophins in organotypic slices of developing cortex with Trk receptor bodies (Trk-lgGs), we found that BDNF and NT-3 oppose one another in regulating the dendritic growth of pyramidal neurons. In layer 4, both endogenous and exogenous NT-3 inhibited the dendritic growth stimulated by BDNF. In contrast, in layer 6 both endogenous and exogenous BDNF inhibited dendritic growth stimulated by NT-3. These antagonistic actions of endogenous BDNF and NT-3 provide a mechanism by which dendritic growth and retraction can be dynamically regulated during cortical development, and suggest that the multiple neurotrophins expressed in developing cortex represent distinct components of an extracellular signaling system for regulating dendritic growth.