Breast milk-derived human milk oligosaccharides promote Bifidobacterium interactions within a single ecosystem
Breast milk-derived human milk oligosaccharides promote Bifidobacterium interactions within a single ecosystem
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DOI：
10.1038/s41396-019-0553-2
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发表时间：
2020-02-01
期刊：
影响因子：
11
通讯作者：
Hall, Lindsay J.
中科院分区：
文献类型：
--
作者：
Lawson, Melissa A. E.;O'Neill, Ian J.;Hall, Lindsay J.
饮食与微生物的相互作用在调节早期微生物群方面发挥着重要作用，其中双歧杆菌菌株和物种在母乳喂养婴儿的肠道中占主导地位。在这里，我们试图探索婴儿饮食如何驱动不同的双歧杆菌细菌群落组成和动态在个别婴儿生态系统。从母乳喂养的婴儿中分离的19种菌株的基因组特征揭示了富含碳水化合物代谢基因的多样化基因组结构，这对每种菌株都是不同的，但在婴儿中共同形成了泛基因组。与母乳低聚糖（HMO）消化有关的基因簇的存在在物种之间存在差异，生长研究表明，在单个婴儿中，菌株之间利用2 ' FL和LNnT HMO的能力存在差异。用HMO降解剂和非HMO使用者进行交叉喂养实验（使用用过的或“条件”培养基和直接共培养）。进一步的H-1-NMR分析鉴定了岩藻糖、半乳糖、乙酸盐和N-乙酰葡糖胺作为HMO代谢的关键副产物;如通过非HMO使用者对来自HMO代谢的消耗培养基的适度增长所证明的。这些实验表明HMO代谢如何允许资源共享，以最大限度地提高饮食中的营养消耗，并突出了双歧杆菌菌株的合作性质及其作为婴儿生态系统中“基础”物种的作用。婴儿内和婴儿间双歧杆菌群落的行为可能有助于双歧杆菌在生命早期的多样性和优势地位，并为未来开发新的饮食和基于微生物群的疗法以促进婴儿健康提供了途径。
Diet-microbe interactions play an important role in modulating the early-life microbiota, with Bifidobacterium strains and species dominating the gut of breast-fed infants. Here, we sought to explore how infant diet drives distinct bifidobacterial community composition and dynamics within individual infant ecosystems. Genomic characterisation of 19 strains isolated from breast-fed infants revealed a diverse genomic architecture enriched in carbohydrate metabolism genes, which was distinct to each strain, but collectively formed a pangenome across infants. Presence of gene clusters implicated in digestion of human milk oligosaccharides (HMOs) varied between species, with growth studies indicating that within single infants there were differences in the ability to utilise 2 ' FL and LNnT HMOs between strains. Cross-feeding experiments were performed with HMO degraders and non-HMO users (using spent or 'conditioned' media and direct co-culture). Further H-1-NMR analysis identified fucose, galactose, acetate, and N-acetylglucosamine as key by-products of HMO metabolism; as demonstrated by modest growth of non-HMO users on spend media from HMO metabolism. These experiments indicate how HMO metabolism permits the sharing of resources to maximise nutrient consumption from the diet and highlights the cooperative nature of bifidobacterial strains and their role as 'foundation' species in the infant ecosystem. The intra- and inter-infant bifidobacterial community behaviour may contribute to the diversity and dominance of Bifidobacterium in early life and suggests avenues for future development of new diet and microbiota-based therapies to promote infant health.