Aligned electrospun cellulose scaffolds coated with rhBMP-2 for both in vitro and in vivo bone tissue engineering
Aligned electrospun cellulose scaffolds coated with rhBMP-2 for both in vitro and in vivo bone tissue engineering
复制标题
涂有 rhBMP-2 的对齐电纺纤维素支架，用于体外和体内骨组织工程
DOI：
10.1016/j.carbpol.2019.02.038
复制
发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Ping Ji
中科院分区：
文献类型：
--
作者：
Ximu Zhang;Chao Wang;Min Liao;Lina Dai;Yingying Tang;Hongmei Zhang;Phil Coates;Farshid Sefat;Liwen Zheng;Jinlin Song;Zhuo Zheng;Dan Zhao;Maobin Yang;Wei Zhang;Ping Ji
纳米纤维和排列结构等支架材料的物理性质对干细胞的生长和分化有着重要的影响，这主要是由于它们具有归巢效应和接触导向作用。然而，用于骨支架的对齐的纳米纤维的生物学功能尚未被严格表征。在本研究中，定向电纺纤维素/CNCs纳米复合纳米纤维（ECCNNs）负载骨形态发生蛋白-2（BMP-2）首次用于研究（1）人骨髓间充质干细胞（BMSCs）体外成骨分化和（2）体内胶原组装方向和皮质骨再生。负载BMP-2的ECCNNs支架具有良好的生物相容性。体外培养BMSCs的生长方向遵循基本的排列整齐的细胞形态，并伴有茜素红染色、碱性磷酸酶（ALP）活性和钙含量的增加，而在体内研究中，使用兔颅骨骨缺损模型进行显微CT和组织学分析。
Physical properties of scaffolds such as nanofibers and aligned structures have been reported to exert profound effects on the growth and differentiation of stem cells due to their homing-effect features and contact guidance. However, the biological function of aligned nanofibers utilized as bone-scaffold has not been rigorously characterized. In the present study, aligned electrospun cellulose/CNCs nanocomposite nanofibers (ECCNNs) loaded with bone morphogenic protein-2 (BMP-2) were used for the first time to investigate (1) in vitro osteogenic differentiation of human mesenchymal stem cells (BMSCs) and (2) in vivo collagen assembly direction and cortical bone regeneration. Aligned ECCNNs scaffolds loaded with BMP-2 possess good biological compatibility. The growth orientation of BMSCs followed the underlying aligned nanofiber morphology, accompanied with increased alizarin red stain, alkaline phosphatase (ALP) activity and calcium content in vitro while, a rabbit calvaria bone defect model was used in an in vivo study with micro CT and histology analyses.