Targeted inactivation of antibiotic-resistant Escherichia coli and Pseudomonas aeruginosa in a soil-lettuce system by combined polyvalent bacteriophage and biochar treatment
Targeted inactivation of antibiotic-resistant Escherichia coli and Pseudomonas aeruginosa in a soil-lettuce system by combined polyvalent bacteriophage and biochar treatment
复制标题
通过多价噬菌体和生物炭联合处理，有针对性地灭活土壤-生菜系统中的抗生素耐药性大肠杆菌和铜绿假单胞菌
DOI：
10.1016/j.envpol.2018.04.070
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发表时间：
2018-10-01
影响因子：
8.9
通讯作者：
Hu, Feng
中科院分区：
文献类型：
--
作者：
Ye, Mao;Sun, Mingming;Hu, Feng
农业土壤-植物系统中高丰度的耐药病原菌（ARPB）和抗生素抗性基因（ARGs）已严重威胁人类健康和环境安全。因此，开发有针对性的技术来控制土壤-植物系统中现有的抗生素抗性（AR）污染和潜在的传播至关重要。在这项工作中，多价噬菌体（噬菌体）治疗和生物炭修正案分别和组合，以刺激ARPB/ARG消散在土壤生菜系统。生物质炭和多价噬菌体联合施用后，培养63 d后，土壤中大肠杆菌K-12（泰特（R））和铜绿假单胞菌PAO 1（amp（R）+ fos（R））及其相应的ARG（tetM、tetQ、tetW、ampC和fosA）的丰度显著降低（p < 0.05）。内生K-12和PAO 1，和ARGs，也得到了类似的结果，在莴苣组织后组合处理。此外，高通量测序显示，生物炭和多价噬菌体协同提高土壤中的土著细菌群落和生菜中的内生细菌群落的结构多样性和功能稳定性。因此，这项工作提出了一种新的生物技术，结合生物炭修正和多价噬菌体治疗，以实现有针对性的ARPB，刺激ARG消散在土壤生菜系统的失活。(C)2018爱思唯尔有限公司版权所有
High abundances of antibiotic-resistant pathogenic bacteria (ARPB) and antibiotic resistance genes (ARGs) in agricultural soil-plant systems have become serious threats to human health and environmental safety. Therefore, it is crucial to develop targeted technology to control existing antibiotic resistance (AR) contamination and potential dissemination in soil-plant systems. In this work, polyvalent bacteriophage (phage) therapy and biochar amendment were applied separately and in combination to stimulate ARPB/ARG dissipation in a soil-lettuce system. With combined application of biochar and polyvalent phage, the abundance of Escherichia coli K-12 (tet(R)) and Pseudomonas aeruginosa PAO1 (amp(R) + fos(R)) and their corresponding ARGs (tetM, tetQ, tetW, ampC, and fosA) significantly decreased in the soil after 63 days' incubation (p < 0.05). Similar results for endophytic K-12 and PAO1, and ARGs, were also obtained in lettuce tissues following combined treatment. Additionally, high throughput sequencing revealed that biochar and polyvalent phage synergetically improved the structural diversity and functional stability of the indigenous bacterial communities in soil and the endophytic ones in lettuce. Hence, this work proposes a novel biotechnology that combines biochar amendment and polyvalent phage therapy to achieve targeted inactivation of ARPB, which stimulates ARG dissipation in soil-lettuce systems. (C) 2018 Elsevier Ltd. All rights reserved.