Identification of omentin mRNA in human epicardial adipose tissue: comparison to omentin in subcutaneous, internal mammary artery periadventitial and visceral abdominal depots
Identification of omentin mRNA in human epicardial adipose tissue: comparison to omentin in subcutaneous, internal mammary artery periadventitial and visceral abdominal depots
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DOI：
10.1038/sj.ijo.0803790
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发表时间：
2008-05-01
影响因子：
4.9
通讯作者：
Madan, A. K.
中科院分区：
文献类型：
--
作者：
Fain, J. N.;Sacks, H. S.;Madan, A. K.
目的：本研究旨在确定人心外膜、内周乳、上胸、上腹部和腿部静脉皮下脂肪组织中大网膜素和内脏脂肪素mRNA的相对分布，以及大网膜脂肪组织中非脂肪细胞和脂肪细胞中大网膜蛋白的分布。背景：大网膜中存在大网膜蛋白，但皮下脂肪中不存在大网膜蛋白。大网膜素和内脏脂肪素被认为是内脏腹部脂肪的标志物。研究设计和方法：通过对接受心脏或减肥手术的人体脂肪样本进行qRT-PCR分析来测量大网膜素和内脏脂肪素的mRNA含量。结果：内乳脂肪中大网膜素mRNA为3.5%，上胸段皮下脂肪中大网膜素mRNA为4.7%，而其他皮下脂肪库中大网膜素mRNA含量不到1%。心外膜脂肪中的网膜素。内脏脂肪素 mRNA 的分布在五个储存库之间没有变化。大网膜脂肪组织的非脂肪细胞中优先表达大网膜蛋白mRNA，因为分离的脂肪细胞中大网膜蛋白mRNA的含量是非脂肪细胞中的9%，内脂素也有类似的结果。分化脂肪细胞中网膜蛋白mRNA的量为非脂肪细胞中的0.3％，内脂素mRNA的量为4％。前脂肪细胞中的网膜素 mRNA 含量几乎检测不到，而内脂素的含量仅为网膜脂肪组织新鲜分离的非脂肪细胞中的 3%。结论：大网膜蛋白 mRNA 主要存在于人类心外膜和大网膜脂肪中，而内脂素 mRNA 在心外膜、皮下和网膜脂肪中也有相同程度的发现。
Objective: The purpose of this study was to determine the relative distribution of omentin and visfatin mRNA in human epicardial, peri-internal mammary, upper thoracic, upper abdominal and leg vein subcutaneous adipose tissue as well as the distribution of omentin in the nonfat cells and adipocytes of human omental adipose tissue.Background: Omentin is found in human omentum but not subcutaneous fat. Omentin and visfatin are considered markers of visceral abdominal fat.Research design and methods: The mRNA content of omentin and visfatin was measured by qRT-PCR analysis of fat samples removed from humans undergoing cardiac or bariatric surgery.Results: Omentin mRNA in internal mammary fat was 3.5%, that in the upper thoracic subcutaneous fat was 4.7% while that in the other subcutaneous fat depots was less than 1% of omentin in epicardial fat. The distribution of visfatin mRNA did not vary between the five depots. Omentin mRNA was preferentially expressed in the nonfat cells of omental adipose tissue since the omentin mRNA content of isolated adipocytes was 9% of that in nonfat cells, and similar results were seen for visfatin. The amount of omentin mRNA in differentiated adipocytes was 0.3% and that of visfatin 4% of that in nonfat cells. The amount of omentin mRNA in preadipocytes was virtually undetectable while that of visfatin was 3% of that in freshly isolated nonfat cells from omental adipose tissue.Conclusion: Omentin mRNA is predominantly found in epicardial and omental human fat whereas visfatin mRNA is found to the same extent in epicardial, subcutaneous and omental fat.