Variations in polyoma virus genotype in relation to tumor induction in mice. Characterization of wild type strains with widely differing tumor profiles.
Variations in polyoma virus genotype in relation to tumor induction in mice. Characterization of wild type strains with widely differing tumor profiles.
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发表时间：
1987-05
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通讯作者：
Clyde J. Dawe;R. Freund;Gail Mandel;Kurt BALLMER-HOFER;D. A. Talmage;Thomas L. Benjamin
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作者：
Clyde J. Dawe;R. Freund;Gail Mandel;Kurt BALLMER-HOFER;D. A. Talmage;Thomas L. Benjamin
作者探讨了小鼠多瘤病毒基因型变异对小鼠肿瘤形成模式的影响。调查了四种“野生型”病毒株。两个是高度致癌的，诱导多个肿瘤的上皮和间质起源，在高频率和短潜伏期。另外两种菌株是弱致癌性的，诱导较少的肿瘤，仅为间充质来源，并且经过长时间的潜伏期。这些鲜明对比的肿瘤特征是用来自分子克隆病毒基因组的病毒原种复制的。虽然它们的致癌特性有很大的不同，但这些克隆病毒在转化培养的大鼠成纤维细胞方面被证明是同样有效的，并且在培养的小鼠细胞中显示出相同的肿瘤抗原表达模式。复合物的多瘤中T抗原和pp 60 c-src的上皮肿瘤的提取物中显示的高致癌性病毒株诱导。它的结论是，多瘤病毒的遗传决定因素在小鼠中的肿瘤诱导比以前定义的细胞转化系统的使用更复杂。
The authors have explored the effects of variations in mouse polyoma virus genotype on patterns of tumor formation in the mouse. Four "wild type" virus strains were surveyed. Two were highly oncogenic, inducing multiple tumors of epithelial and mesenchymal origin, at high frequency and with short latency. The other two strains were weakly oncogenic, inducing fewer tumors, solely of mesenchymal origin, and after a long latency. These sharply contrasting tumor profiles were reproduced with virus stocks derived from molecularly cloned viral genomes. Though vastly different in their oncogenic properties, these cloned viruses proved equally effective in transforming established rat fibroblasts in culture and showed the same patterns of tumor antigen expression in cultured mouse cells. Complexes of polyoma middle T antigen and pp60c-src were demonstrated in extracts of epithelial tumors induced by a highly oncogenic virus strain. It is concluded that polyoma viral genetic determinants for tumor induction in the mouse are more complex than those previously defined by the use of cell transformation systems.