THE TRANSCRIPTION UNIT OF THE DROSOPHILA-ENGRAILED LOCUS - AN UNUSUALLY SMALL PORTION OF A 70-000 BP GENE
THE TRANSCRIPTION UNIT OF THE DROSOPHILA-ENGRAILED LOCUS - AN UNUSUALLY SMALL PORTION OF A 70-000 BP GENE
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DOI：
10.1002/j.1460-2075.1987.tb02576.x
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发表时间：
1987-09-01
期刊：
影响因子：
11.4
通讯作者：
KORNBERG, T
中科院分区：
文献类型：
--
作者：
DREES, B;ALI, Z;KORNBERG, T
已通过北方、S1核酸酶敏感性和引物延伸分析表征了来自黑腹果蝇的enrailed基因的转录物。该嵌入基因编码三种poly（A）+转录物（3.6 kg、2.7 kb和1.4 kb），这些转录物源自基因组的3.9 kb部分。没有发现其他转录区域高达16 kb的下游和48 kb的上游的盘根错节的转录单位，该基因组的一部分，其中盘根错节的突变已被映射。在转录沉默区的点上破坏基因座的致死性错切突变不影响错切转录物的结构。通过北方分析还鉴定了围绕着所述有爪的基因座的145 kb DNA所表达的转录物。与大部分转录不活跃的被植入基因相反，大部分周围区域的转录本密度高出10倍。我们推测，异常大的沉默区在周围的盘根错节的转录单位表示存在的特殊机制，调节其表达。
Transcripts from the engrailed gene of Drosophila melanogaster have been characterized by Northern, S1 nuclease sensitivity, and primer extension analyses. The engrailed gene encodes three poly(A)+ transcripts (3.6 kg, 2.7 kb, and 1.4 kb) that derive from a 3.9-kb portion of the genome. No other transcribed regions were found up to 16 kb downstream and 48 kb upstream of the engrailed transcription unit, the portion of the genome to which engrailed mutations have been mapped. The structures of the engrailed transcripts are unaffected by lethal engrailed mutations that break the locus at points in the transcriptionally silent regions. Transcripts expressed by 145 kb of DNA that surround the engrailed locus were also identified by Northern analysis. In contrast to the large portion of the engrailed gene that is transcriptionally inactive, most of the surrounding regions are 10-fold more densely populated with transcripts. We presume that the unusually large silent region at the periphery of the engrailed transcription unit signify the presence of special mechanisms that regulate its expression.