Immunohistochemical localization of placental leucine aminopeptidase/oxytocinase in normal human placental, fetal and adult tissues
Immunohistochemical localization of placental leucine aminopeptidase/oxytocinase in normal human placental, fetal and adult tissues
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DOI：
10.1071/r97055
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发表时间：
1997-01-01
影响因子：
1.9
通讯作者：
Mizutani, S
中科院分区：
文献类型：
--
作者：
Nagasaka, T;Nomura, S;Mizutani, S
虽然已知催产素酶存在于妊娠血清和胎盘中，但本研究描述了该酶的 mRNA 在多种其他人体组织中的表达。 Northern 印迹分析用于检测 mRNA，探针源自催产素酶/胎盘亮氨酸氨基肽酶 (P-LAP) 的 cDNA。免疫反应性催产素酶/P-LAP 蛋白的分布和定位均已通过使用抗 P-LAP 抗体在正常胎盘、胎儿和成人组织中通过免疫组织化学测定。在胎盘组织中，仅合体滋养层呈阳性染色。在胎儿和成人组织中，血管内皮细胞、胃肠粘膜细胞、肝胆、胰胆、支气管肺泡和肾小管系统的上皮细胞以及胰腺的胰岛细胞和中枢神经系统的神经元均获得阳性染色。成人的汗腺细胞、精囊和前列腺，以及胎儿的脂肪细胞和骨骼肌细胞也被染色。 P-LAP 的广泛分布表明它参与多种生理事件，不仅限于调节妊娠期精氨酸加压素 (AVP) 和催产素 (OT) 等生物活性肽的量。合体滋养细胞中 P-LAP 的存在支持了妊娠血清中 P-LAP 源自胎盘的观点。
While oxytocinase is known to exist in pregnancy serum and placenta, the present study describes the expression of the mRNA for this enzyme in a wide variety of other human tissues. Northern blot analysis was used to detect the mRNA, with a probe derived from a cDNA for oxytocinase/placental leucine aminopeptidase (P-LAP). Both the distribution and localization of immunoreactive oxytocinase/P-LAP protein have been determined immunohistochemically by use of an anti-P-LAP antibody in normal placental, fetal and adult tissues. In placental tissues, only syncytiotrophoblasts were stained positively. In both fetal and adult tissues, positive staining was obtained in vascular endothelial cells, gastrointestinal mucosal cells, epithelial cells of hepato-biliary, pancreato-biliary, bronchial-alveolar and renal tubular systems as well as islet cells of pancreas and neurons in the central nervous systems. Sweat-gland cells, seminal vesicles and prostate gland in the adult, as well as adipocytes and skeletal muscle cells in the fetus were also stained. The widespread distribution of P-LAP suggests its involvement in a variety of physiological events not restricted to the regulation of the amounts of bioactive peptides such as arginine vasopressin (AVP) and oxytocin (OT) in pregnancy. The presence of P-LAP in syncytiotrophoblasts supports the idea that P-LAP in pregnancy serum is derived from the placenta.